Active Recombinant Human DDR2(467-end) Protein, N-His-tagged

• Cat.No.: DDR2-56H
• Product Overview: N-terminal 6Histagged, recombinant, human DDR2 amino acids 467–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: DDR2 is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 467-end
• Form: 1.595mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 33U/mg, where one unit of DDR2, active activity is defined as 1nmol phosphate incorporated into 250μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 48 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMSLRL GSSNSTYDRI FPLRPDYQEP SRLIRKLPEF 61 APGEEESGCS GVVKPVQPSG PEGVPHYAEA DIVNLQGVTG GNTYSVPAVT MDLLSGKDVA 121 VEEFPRKLLT FKEKLGEGQF GEVHLCEVEG MEKFKDKDFA LDVSANQPVL VAVKMLRADA 181 NKNARNDFLK EIKIMSRLKD PNIIHLLAVC ITDDPLCMIT EYMENGDLNQ FLSRHEPPNS 241 SSSDVRTVSY TNLKFMATQI ASGMKYLSSL NFVHRDLATR NCLVGKNYTI KIADFGMSRN 301 LYSGDYYRIQ GRAVLPIRWM SWESILLGKF TTASDVWAFG VTLWETFTFC QEQPYSQLSD 361 EQVIENTGEF FRDQGRQTYL PQPAICPDSV YKLMLSCWRR DTKNRPSFQE IHLLLLQQGD 421 E
• Purity: Purity 57.3% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: DDR2 discoidin domain receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: DDR2
• Synonyms: DDR2; discoidin domain receptor tyrosine kinase 2; TKT; WRCN; MIG20a; NTRKR3; TYRO10
• Gene ID: 4921
• mRNA Refseq: NM_001014796.3
• Protein Refseq: NP_001014796.1
• MIM: 191311
• UniProt ID: Q16832