| Description : |
Glycogen synthase kinase 3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. There exists two isoforms of GSK3, GSK3A and GSK3B, and they are strictly regulated via phosphorylation. GSK3 has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3A also has been inhibited by activation of the canonical Wnt pathway, resulting in increased catenin that drives human mesenchymal stem cells to osteoblasts. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
2-end |
| Form : |
0.692mg/ml of enzyme in 50mM Tris/HCl, pH7.5, 270mM sucrose, 0.1mM EGTA, 150mM NaCl, 1mM benzamidine, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1005U/mg, where one unit of GSK-3α, active activity is defined as 1nmol phosphate incorporated into 200μM GSK-3 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
55 kDa |
| AA Sequence : |
1 MHHHHHHEFS GGGPSGGGPG GSGRARTSSF AEPGGGGGGG GGGPGGSASG PGGTGGGKAS 61 VGAMGGGVGA SSSGGGPGGS GGGGSGGPGA GTSFPPPGVK LGRDSGKVTT VVATLGQGPE 121 RSQEVAYTDI KVIGNGSFGV VYQARLAETR ELVAIKKVLQ DKRFKNRELQ IMRKLDHCNI 181 VRLRYFFYSS GEKKDELYLN LVLEYVPETV YRVARHFTKA KLTIPILYVK VYMYQLFRSL 241 AYIHSQGVCH RDIKPQNLLV DPDTAVLKLC DFGSAKQLVR GEPNVSYICS RYYRAPELIF 301 GATDYTSSID VWSAGCVLAE LLLGQPIFPG DSGVDQLVEI IKVLGTPTRE QIREMNPNYT 361 EFKFPQIKAH PWTKVFKSRT PPEAIALCSS LLEYTPSSRL SPLEACAHSF FDELRCLGTQ 421 LPNNRPLPPL FNFSAGELSI QPSLNAILIP PHLRSPAGTT TLTPSSQALT ETPTSSDWQS 481 TDATPTLTNS S |
| Purity : |
Purity 59.5% by testing method. |
| Applications : |
Drug Discovery & Development |
| Storage : |
1 year at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
