Active Recombinant Human HIPK3(161-562) Protein, N-His-tagged

• Cat.No.: HIPK3-102H
• Product Overview: N-terminal 6Histagged, recombinant, human HIPK3, amino acids 161–562, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: Homeodomain-interacting protein kinase 3 (HIPK3), a serine/threonine kinase, has been described as a member of a family of nuclear kinases that act as co-repressors for homeodomain transcription factors. HIPK3 can activate p53-mediated transcriptional regulation of MDM2 and antagonize Myc/Ras-induced transformation. HIPK3 may play an important role in the regulation of p53 apoptotic function. Inhibition of HIPK3 may inhibit apoptosis and facilitate tumorigenesis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 161-562 aa
• Form: 1.65mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 747U/mg, where one unit of HIPK3, active activity is defined as 1nmol phosphate incorporated into 1mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 49.7kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF PVTVVTATTG SKQNCTTGEG DYQLVQHEVL 61 CSMKNTYEVL DFLGRGTFGQ VVKCWKRGTN EIVAIKILKN HPSYARQGQI EVSILARLST 121 ENADEYNFVR AYECFQHRNH TCLVFEMLEQ NLYDFLKQNK FSPLPLKVIR PILQQVATAL 181 KKLKSLGLIH ADLKPENIML VDPVRQPYRV KVIDFGSASH VSKTVCSTYL QSRYYRAPEI 241 ILGLPFCEAI DMWSLGCVIA ELFLGWPLYP GALEYDQIRY ISQTQGLPGE QLLNVGTKST 301 RFFCKETDMS HSGWRLKTLE EHEAETGMKS KEARKYIFNS LDDVAHVNTV MDLEGSDLLA 361 EKADRREFVS LLKKMLLIDA DLRITPAETL NHPFVNMKHL LDFPHSNHVK SCFHIMDICK 421 SHLNSCDTNN HN
• Purity: Purity 93.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: HIPK3 homeodomain interacting protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: HIPK3
• Synonyms: HIPK3; homeodomain interacting protein kinase 3; PKY; YAK1; DYRK6; FIST3
• Gene ID: 10114
• mRNA Refseq: NM_005734.5
• Protein Refseq: NP_005725.3
• MIM: 604424
• UniProt ID: Q9H422