Active Recombinant Human HK1 Protein, His-tagged

• Cat.No.: HK1-01H
• Product Overview: Recombinant human Hexokinase1 (1-917aa), fused to His-tag at Nterminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: > 8,000pmol/min/μg. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH 7.5 at 37 centigrade.
• Molecular Mass: 104.6 kDa (937aa)
• AA Sequence: MIAAQLLAYYFTELKDDQVKKIDKY LYAMRLSDETLIDIMTRFRKEMKNG LSRDFNPTATVKMLPTFVRSIPDGS EKGDFIALDLGGSSFRILRVQVNHE KNQNVHMESEVYDTPENIVHGSGSQ LFDHVAECLGDFMEKRKIKDKKLPV GFTFSFPCQQSKIDEAILITWTKRF KASGVEGADVVKLLNKAIKKRGDYD ANIVAVVNDTVGTMMTCGYDDQHCE VGLIIGTGTNACYMEELRHIDLVEG DEGRMCINTEWGAFGDDGSLEDIRT EFDREIDRGSLNPGKQLFEKMVSGM YLGELVRLILVKMAKEGLLFEGRIT PELLTRGKFNTSDVSAIEKNKEGLH NAKEILTRLGVEPSDDDCVSVQHVC TIVSFRSANLVAATLGAILNRLRDN KGTPRLRTTVGVDGSLYKTHPQYSR RFHKTLRRLVPDSDVRFLLSESGSG KGAAMVTAVAYRLAEQHRQIEETLA HFHLTKDMLLEVKKRMRAEMELGLR KQTHNNAVVKMLPSFVRRTPDGTEN GDFLALDLGGTNFRVLLVKIRSGKK RTVEMHNKIYAIPIEIMQGTGEELF DHIVSCISDFLDYMGIKGPRMPLGF TFSFPCQQTSLDAGILITWTKGFKA TDCVGHDVVTLLRDAIKRREEFDLD VVAVVNDTVGTMMTCAYEEPTCEVG LIVGTGSNACYMEEMKNVEMVEGDQ GQMCINMEWGAFGDNGCLDDIRTHY DRLVDEYSLNAGKQRYEKMISGMYL GEIVRNILIDFTKKGFLFRGQISET LKTRGIFETKFLSQIESDRLALLQV RAILQQLGLNSTCDDSILVKTVCGV VSRRAAQLCGAGMAAVVDKIRENRG LDRLNVTVGVDGTLYKLHPHFSRIM HQTVKELSPKCNVSFLLSEDGSGKG AALITAVGVRLRTEASS
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Gene Information

• Gene Name: HK1 hexokinase 1 [ Homo sapiens (human) ]
• Official Symbol: HK1
• Synonyms: HK1; hexokinase 1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; NEDVIBA; hexokinase; hexokinase-1; brain form hexokinase; glycolytic enzyme; hexokinase IR; hexokinase type I; hexokinase-A; EC 2.7.1.1
• Gene ID: 3098
• mRNA Refseq: NM_000188
• Protein Refseq: NP_000179
• MIM: 142600
• UniProt ID: P19367