Active Recombinant Human MELK(1-340) Protein, N-GST-tagged

• Cat.No.: MELK-136H
• Product Overview: N-terminal GSTtagged, recombinant, human MELK amino acids 1–340, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: Recombinant human MELK (amino acids 1 - 340), containing an N-terminal GST tag. Maternal embryonic leucine zipper kinase (MELK) is a conserved serine/threonine kinase involved in a variety of cellular functions, including proliferation, differentiation and apoptosis. Its levels are elevated in a number of tumor types and it may be a prognosis marker for some cancers.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-340 aa
• Form: 1.644mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3053U/mg, where one unit of MELK activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 66 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MKDYDELLKY 241 YELHETIGTG GFAKVKLACH ILTGEMVAIK IMDKNTLGSD LPRIKTEIEA LKNLRHQHIC 301 QLYHVLETAN KIFMVLEYCP GGELFDYIIS QDRLSEEETR VVFRQIVSAV AYVHSQGYAH 361 RDLKPENLLF DEYHKLKLID FGLCAKPKGN KDYHLQTCCG SLAYAAPELI QGKSYLGSEA 421 DVWSMGILLY VLMCGFLPFD DDNVMALYKK IMRGKYDVPK WLSPSSILLL QQMLQVDPKK 481 RISMKNLLNH PWIMQDYNYP VEWQSKNPFI HLDDDCVTEL SVHHRNNRQT MEDLISLWQY 541 DHLTATYLLL LAKKARGKPV RLRLSSFSCG
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MELK maternal embryonic leucine zipper kinase [ Homo sapiens (human) ]
• Official Symbol: MELK
• Synonyms: MELK; maternal embryonic leucine zipper kinase; HPK38
• Gene ID: 9833
• mRNA Refseq: NM_014791.4
• Protein Refseq: NP_055606.1
• MIM: 607025
• UniProt ID: Q14680