Active Recombinant Human MTOR(1362-end) Protein, N-Flag-tagged

• Cat.No.: MTOR-167H
• Product Overview: N-terminal FLAGTM - tagged, recombinant, human mTOR, amino acids 1362–end, expressed by baculovirus in Sf21 insect cells. Purified using FLAGTM agarose.

Specification

• Description: mTOR (Mechanistic Target Of Rapamycin Kinase) belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections.
• Source: Sf21
• Species: Human
• Tag: Flag
• Protein length: 1362-end
• Form: 0.29mg/ml of enzyme in 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300μg/ml FLAGTM peptide, 20% v/v glycerol.
• Bio-activity: 186U/mg, where one unit of mTOR, active activity is defined as 1nmole of phosphate incorporated into 2mg/ml mTOR substrate (cat#. 12-645-K) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 137.3 kDa
• AA Sequence: 1 MDYKDDDDKE FMEHSDKGPL PLRDDNGIVL LGERAAKCRA YAKALHYKEL EFQKGPTPAI 61 LESLISINNK LQQPEAAAGV LEYAMKHFGE LEIQATWYEK LHEWEDALVA YDKKMDTNKD 121 DPELMLGRMR CLEALGEWGQ LHQQCCEKWT LVNDETQAKM ARMAAAAAWG LGQWDSMEEY 181 TCMIPRDTHD GAFYRAVLAL HQDLFSLAQQ CIDKARDLLD AELTAMAGES YSRAYGAMVS 241 CHMLSELEEV IQYKLVPERR EIIRQIWWER LQGCQRIVED WQKILMVRSL VVSPHEDMRT 301 WLKYASLCGK SGRLALAHKT LVLLLGVDPS RQLDHPLPTV HPQVTYAYMK NMWKSARKID 361 AFQHMQHFVQ TMQQQAQHAI ATEDQQHKQE LHKLMARCFL KLGEWQLNLQ GINESTIPKV 421 LQYYSAATEH DRSWYKAWHA WAVMNFEAVL HYKHQNQARD EKKKLRHASG ANITNATTAA 481 TTAATATTTA STEGSNSESE AESTENSPTP SPLQKKVTED LSKTLLMYTV PAVQGFFRSI 541 SLSRGNNLQD TLRVLTLWFD YGHWPDVNEA LVEGVKAIQI DTWLQVIPQL IARIDTPRPL 601 VGRLIHQLLT DIGRYHPQAL IYPLTVASKS TTTARHNAAN KILKNMCEHS NTLVQQAMMV 661 SEELIRVAIL WHEMWHEGLE EASRLYFGER NVKGMFEVLE PLHAMMERGP QTLKETSFNQ 721 AYGRDLMEAQ EWCRKYMKSG NVKDLTQAWD LYYHVFRRIS KQLPQLTSLE LQYVSPKLLM 781 CRDLELAVPG TYDPNQPIIR IQSIAPSLQV ITSKQRPRKL TLMGSNGHEF VFLLKGHEDL 841 RQDERVMQLF GLVNTLLAND PTSLRKNLSI QRYAVIPLST NSGLIGWVPH CDTLHALIRD 901 YREKKKILLN IEHRIMLRMA PDYDHLTLMQ KVEVFEHAVN NTAGDDLAKL LWLKSPSSEV 961 WFDRRTNYTR SLAVMSMVGY ILGLGDRHPS NLMLDRLSGK ILHIDFGDCF EVAMTREKFP 1021 EKIPFRLTRM LTNAMEVTGL DGNYRITCHT VMEVLREHKD SVMAVLEAFV YDPLLNWRLM 1081 DTNTKGNKRS RTRTDSYSAG QSVEILDGVE LGEPAHKKTG TTVPESIHSF IGDGLVKPEA 1141 LNKKAIQIIN RVRDKLTGRD FSHDDTLDVP TQVELLIKQA TSHENLCQCY IGWCPFW
• Purity: Purity 55% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MTOR mechanistic target of rapamycin kinase [ Homo sapiens (human) ]
• Official Symbol: MTOR
• Synonyms: MTOR; mechanistic target of rapamycin kinase; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• Gene ID: 2475
• mRNA Refseq: NM_004958.4
• Protein Refseq: NP_004949.1
• MIM: 601231
• UniProt ID: P42345