Active Recombinant Human PAK5(425-end) Protein, N-His-tagged

• Cat.No.: PAK5-181H
• Product Overview: N-terminal 6Histagged, recombinant, human PAK5, amino acids 425–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: PAK5 is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 425-end
• Form: 2.067mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2565U/mg, where one unit of PAK5 activity is defined as 1nmol phosphate incorporated into PAKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 37.4 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SRVSHEQFRA ALQLVVSPGD PREYLANFIK 61 IGEGSTGIVC IATEKHTGKQ VAVKKMDLRK QQRRELLFNE VVIMRDYHHD NVVDMYSSYL 121 VGDELWVVME FLEGGALTDI VTHTRMNEEQ IATVCLSVLR ALSYLHNQGV IHRDIKSDSI 181 LLTSDGRIKL SDFGFCAQVS KEVPKRKSLV GTPYWMAPEV ISRLPYGTEV DIWSLGIMVI 241 EMIDGEPPYF NEPPLQAMRR IRDSLPPRVK DLHKVSSVLR GFLDLMLVRE PSQRATAQEL 301 LGHPFLKLAG PPSCIVPLMR QYRHH
• Purity: Purity 95.8% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PAK5 p21 (RAC1) activated kinase 5 [ Homo sapiens (human) ]
• Official Symbol: PAK5
• Synonyms: PAK5; p21 (RAC1) activated kinase 5; PAK7
• Gene ID: 57144
• mRNA Refseq: NM_020341.5
• Protein Refseq: NP_065074.1
• MIM: 608038
• UniProt ID: Q9P286