Active Recombinant Human PAK6(382-end) Protein, N-His-tagged

• Cat.No.: PAK6-182H
• Product Overview: N-terminal 6Histagged, recombinant, human PAK6, amino acids 382–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ -NTA agarose.

Specification

• Description: PAK6 is a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. The p21-activated protein kinase 6 gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to co-translocate into the nucleus with AR in response to androgen.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 382-end
• Form: 1.88mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1961U/mg, where one unit of PAK6 activity is defined as 1nmol phosphate incorporated into PAKtide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 37.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF TGVVTHEQFK AALRMVVDQG DPRLLLDSYV 61 KIGEGSTGIV CLAREKHSGR QVAVKMMDLR KQQRRELLFN EVVIMRDYQH FNVVEMYKSY 121 LVGEELWVLM EFLQGGALTD IVSQVRLNEE QIATVCEAVL QALAYLHAQG VIHRDIKSDS 181 ILLTLDGRVK LSDFGFCAQI SKDVPKRKSL VGTPYWMAPE VISRSLYATE VDIWSLGIMV 241 IEMVDGEPPY FSDSPVQAMK RLRDSPPPKL KNSHKVSPVL RDFLERMLVR DPQERATAQE 301 LLDHPFLLQT GLPECLVPLI QLYRKQTSTC
• Purity: Purity 94.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PAK6 p21 (RAC1) activated kinase 6 [ Homo sapiens (human) ]
• Official Symbol: PAK6
• Synonyms: PAK6; p21 (RAC1) activated kinase 6; PAK5
• Gene ID: 56924
• mRNA Refseq: NM_001276717.1
• Protein Refseq: NP_001263646.1
• MIM: 608110
• UniProt ID: Q9NQU5