Active Recombinant Human PIM2(2-end) Protein, N-GST-tagged

• Cat.No.: PIM2-193H
• Product Overview: N-terminal GST-tagged, recombinant, human PIM2, 2–end, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: Protein serine/threonine kinase PIM2 is a member of a small family of highly homologous kinases, including PIM1, PIM2, and PIM3, and all members are expressed in the thymus. Pim plays a role in cell survival, proliferation, differentiation and tumorigenesis. Overexpression of each of these members can promote lymphomagenesis in mice. Inhibition of PIM2 may be useful in the treatment of chronic lymphocytic leukemia (B-CLL) and non-Hodgkin's lymphomas (NHL).
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 2-end
• Form: 0.833mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 107U/mg, where one unit of PIM2, active activity is defined as 1nmol phosphate incorporated into 300μM PIM2tide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.5 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPLG SLTKPLQGPP 241 APPGTPTPPP GGKDREAFEA EYRLGPLLGK GGFGTVFAGH RLTDRLQVAI KVIPRNRVLG 301 WSPLSDSVTC PLEVALLWKV GAGGGHPGVI RLLDWFETQE GFMLVLERPL PAQDLFDYIT 361 EKGPLGEGPS RCFFGQVVAA IQHCHSRGVV HRDIKDENIL IDLRRGCAKL IDFGSGALLH 421 DEPYTDFDGT RVYSPPEWIS RHQYHALPAT VWSLGILLYD MVCGDIPFER DQEILEAELH 481 FPAHVSPDCC ALIRRCLAPK PSSRPSLEEI LLDPWMQTPA EDVTPQPLQR RPCPFGLVLA 541 TLSLAWPGLA PNGQKSHPMA MSQG
• Purity: Purity 36.8% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PIM2 Pim-2 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: PIM2
• Synonyms: PIM2; Pim-2 proto-oncogene, serine/threonine kinase; Pim-2h
• Gene ID: 11040
• mRNA Refseq: NM_006875.4
• Protein Refseq: NP_006866.2
• MIM: 300295
• UniProt ID: Q9P1W9