Active Recombinant Human RET(V804M)(658-end) Mutation Protein, N-GST-tagged

• Cat.No.: RET-212H
• Product Overview: N-Terminal GST-tagged, recombinant, human Ret amino acids 658–end containing the V804M mutation. Expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. The V804M mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC).

Specification

• Description: The RET (rearranged during transfection) receptor tyrosine kinase is a classic example of phenotype heterogeneity. Gain-of-function mutations of RET are associated with human cancer and multiple endocrine neoplasia type 2 (MEN 2A, MEN 2B, etc.) Loss-of-function mutations of RET cause Hirschspung disease or colonic aganglionosis).
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 658-end
• Form: 1.125mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 131U/mg, where one unit of Ret (V804M) activity is defined as 1nmol phosphate incorporated into 100μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 79.2 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQHCYH 241 KFAHKPPISS AEMTFRRPAQ AFPVSYSSSG ARRPSLDSME NQVSVDAFKI LEDPKWEFPR 301 KNLVLGKTLG EGEFGKVVKA TAFHLKGRAG YTTVAVKMLK ENASPSELRD LLSEFNVLKQ 361 VNHPHVIKLY GACSQDGPLL LIMEYAKYGS LRGFLRESRK VGPGYLGSGG SRNSSSLDHP 421 DERALTMGDL ISFAWQISQG MQYLAEMKLV HRDLAARNIL VAEGRKMKIS DFGLSRDVYE 481 EDSYVKRSQG RIPVKWMAIE SLFDHIYTTQ SDVWSFGVLL WEIVTLGGNP YPGIPPERLF 541 NLLKTGHRME RPDNCSEEMY RLMLQCWKQE PDKRPVFADI SKDLEKMMVK RRDYLDLAAS 601 TPSDSLIYDD GLSEEETPLV DCNNAPLPRA LPSTWIENKL YGMSDPNWPG ESPVPLTRAD 661 GTNTGFPRYP NDSVYANWML SPSAAKLMDT FDS
• Purity: Purity 81% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: RET ret proto-oncogene [ Homo sapiens (human) ]
• Official Symbol: RET
• Synonyms: RET; ret proto-oncogene; PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
• Gene ID: 5979
• mRNA Refseq: NM_001355216.2
• Protein Refseq: NP_001342145.1
• MIM: 164761
• UniProt ID: P07949