Active Recombinant Human ROCK1(11-552) Protein, N-His-tagged

• Cat.No.: ROCK1-215H
• Product Overview: N-terminal 6Histagged recombinant human ROCK-II residues 11–552, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Rho-associated, coiled-coil containing protein kinase (ROCK), including the ROCK1 and ROCK2 isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. PKA and the two ROCKs have been reported to have opposite effects on neurite formation. More studies are needed to provide significant insights into the role of ROCK in several pathological processes. ROCK inhibitors have been shown the benefit for treatment of various cancers, inflammation, coronary artery spasms and hypertension.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 11-552 aa
• Form: 1.05mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 814U/mg, where one unit of ROCK-II activity is defined as 1nmol phosphate incorporated into 30μM long S6 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63.3 kDa
• AA Sequence: 1 MHHHHHHPGA PETAPGDGAG ASRQRKLEAL IRDPRSPINV ESLLDGLNSL VLDLDFPALR 61 KNKNIDNFLN RYEKIVKKIR GLQMKAEDYD VVKVIGRGAF GEVQLVRHKA SQKVYAMKLL 121 SKFEMIKRSD SAFFWEERDI MAFANSPWVV QLFYAFQDDR YLYMVMEYMP GGDLVNLMSN 181 YDVPEKWAKF YTAEVVLALD AIHSMGLIHR DVKPDNMLLD KHGHLKLADF GTCMKMDETG 241 MVHCDTAVGT PDYISPEVLK SQGGDGFYGR ECDWWSVGVF LYEMLVGDTP FYADSLVGTY 301 SKIMDHKNSL CFPEDAEISK HAKNLICAFL TDREVRLGRN GVEEIRQHPF FKNDQWHWDN 361 IRETAAPVVP ELSSDIDSSN FDDIEDDKGD VETFPIPKAF VGNQLPFIGF TYYRENLLLS 421 DSPSCRENDS IQSRKNEESQ EIQKKLYTLE EHLSNEMQAK EELEQKCKSV NTRLEKTAKE 481 LEEEITLRKS VESALRQLER EKALLQHKNA EYQRKADHEA DKKRNLENDV NSLKDQLEDL 541 KKRNQNSQI
• Purity: Purity 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ROCK1 Rho associated coiled-coil containing protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ROCK1
• Synonyms: ROCK1; Rho associated coiled-coil containing protein kinase 1; ROCK-I; P160ROCK; MGC131603; p160ROCK; MGC43611; p160-ROCK
• Gene ID: 6093
• mRNA Refseq: NM_005406.3
• Protein Refseq: NP_005397.1
• MIM: 601702
• UniProt ID: Q13464