Active Recombinant Human ROCK1(17-535) Protein, N-His-tagged

• Cat.No.: ROCK1-217H
• Product Overview: N-terminal 6His-tagged, recombinant, human ROCK-I amino acids 17–535, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose. Autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP.

Specification

• Description: Rho-associated, coiled-coil containing protein kinase (ROCK), including the ROCK1 and ROCK2 isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. PKA and the two ROCKs have been reported to have opposite effects on neurite formation. More studies are needed to provide significant insights into the role of ROCK in several pathological processes. ROCK inhibitors have been shown the benefit for treatment of various cancers, inflammation, coronary artery spasms and hypertension.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 17-535 aa
• Form: 0.367mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 63U/mg, where one unit of ROCK-I activity is defined as 1nmol phosphate incorporated into 30μM Long S6 peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 61.4 kDa
• AA Sequence: 1 MHHHHHHLLR DPKSEVNSDC LLDGLDALVY DLDFPALRKN KNIDNFLSRY KDTINKIRDL 61 RMKAEDYEVV KVIGRGAFGE VQLVRHKSTR KVYAMKLLSK FEMIKRSDSA FFWEERDIMA 121 FANSPWVVQL FYAFQDDRYL YMVMEYMPGG DLVNLMSNYD VPEKWARFYT AEVVLALDAI 181 HSMGFIHRDV KPDNMLLDKS GHLKLADFGT CMKMNKEGMV RCDTAVGTPD YISPEVLKSQ 241 GGDGYYGREC DWWSVGVFLY EMLVGDTPFY ADSLVGTYSK IMNHKNSLTF PDDNDISKEA 301 KNLICAFLTD REVRLGRNGV EEIKRHLFFK NDQWAWETLR DTVAPVVPDL SSDIDTSNFD 361 DLEEDKGEEE TFPIPKAFVG NQLPFVGFTY YSNRRYLSSA NPNDNRTSSN ADKSLQESLQ 421 KTIYKLEEQL HNEMQLKDEM EQKCRTSNIK LDKIMKELDE EGNQRRNLES TVSQIEKEKM 481 LLQHRINEYQ RKAEQENEKR RNVENEVSTL KDQLEDLKKV SQNSQL
• Purity: Purity 71.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ROCK1 Rho associated coiled-coil containing protein kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ROCK1
• Synonyms: ROCK1; Rho associated coiled-coil containing protein kinase 1; ROCK-I; P160ROCK; MGC131603; p160ROCK; MGC43611; p160-ROCK
• Gene ID: 6093
• mRNA Refseq: NM_005406.3
• Protein Refseq: NP_005397.1
• MIM: 601702
• UniProt ID: Q13464