Active Recombinant Human SGK3(119-end) Protein, N-His-tagged

• Cat.No.: SGK3-225H
• Product Overview: N-terminal 6Histagged, recombinant, human SGK3 amino acids 119–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: SGK3 is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 119-end
• Form: 0.653mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2582U/mg, where one unit of SGK3, active activity is defined as 1nmol phosphate incorporated into Crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 46.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MDSPKHQSDP SEDEDERSSQ KLHSTSQNIN 61 LGPSGNPHAK PTDFDFLKVI GKGSFGKVLL AKRKLDGKFY AVKVLQKKIV LNRKEQKHIM 121 AERNVLLKNV KHPFLVGLHY SFQTTEKLYF VLDFVNGGEL FFHLQRERSF PEHRARFYAA 181 EIASALGYLH SIKIVYRDLK PENILLDSVG HVVLTDFGLC KEGIAISDTT TTFCGTPEYL 241 APEVIRKQPY DNTVDWWCLG AVLYEMLYGL PPFYCRDVAE MYDNILHKPL SLRPGVSLTA 301 WSILEELLEK DRQNRLGAKE DFLEIQNHPF FESLSWADLV QKKIPPPFNP NVAGPDDIRN 361 FDTAFTEETV PYSVCVSSDY SIVNASVLEA DDAFVGFSYA PPSEDLFL
• Purity: Purity 79.9% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: SGK3 serum/glucocorticoid regulated kinase family member 3 [ Homo sapiens (human) ]
• Official Symbol: SGK3
• Synonyms: SGK3; serum/glucocorticoid regulated kinase 3; CISK; SGK2; SGKL
• Gene ID: 23678
• mRNA Refseq: NM_013257.5
• Protein Refseq: NP_037389.4
• MIM: 607591
• UniProt ID: Q96BR1