Active Recombinant Human WNK3(1-434) Protein, N-His-tagged

• Cat.No.: WNK3-260H
• Product Overview: N-terminal 6Histagged, recombinant, human WNK3, amino acids 1–434. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Members of the 'with no lysine' (WNK) kinase family, such as WNK3, are serine-threonine protein kinases that lack the almost invariant catalytic lysine in subdomain II, which is important for binding ATP in the catalytic site. Instead, these kinases have a conserved lysine in subdomain I that is thought to provide this function.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-434 aa
• Form: 3.36mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 61U/mg, where one unit of WNK3 activity is defined as 1nmol phosphate incorporated into 0.333mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 53.1 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MATDSGDPAS TEDSEKPDGI SFENRVPQVA 61 ATLTVEARLK EKNSTFSASG ETVERKRFFR KSVEMTEDDK VAESSPKDER IKAAMNIPRV 121 DKLPSNVLRG GQEVKYEQCS KSTSEISKDC FKEKNEKEME EEAEMKAVAT SPSGRFLKFD 181 IELGRGAFKT VYKGLDTETW VEVAWCELQD RKLTKAEQQR FKEEAEMLKG LQHPNIVRFY 241 DSWESILKGK KCIVLVTELM TSGTLKTYLK RFKVMKPKVL RSWCRQILKG LQFLHTRTPP 301 IIHRDLKCDN IFITGPTGSV KIGDLGLATL MRTSFAKSVI GTPEFMAPEM YEEHYDESVD 361 VYAFGMCMLE MATSEYPYSE CQNAAQIYRK VTSGIKPASF NKVTDPEVKE IIEGCIRQNK 421 SERLSIRDLL NHAFFAEDTG LRVELAEEDD CSNSSLALRL WVED
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: WNK3 WNK lysine deficient protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: WNK3
• Synonyms: WNK3; WNK lysine deficient protein kinase 3; PRS; MRXS2; PRKWNK3
• Gene ID: 65267
• mRNA Refseq: NM_020922.5
• Protein Refseq: NP_065973.2
• MIM: 300358
• UniProt ID: Q9BYP7