Active Recombinant Mouse ABL1(27-1123) Protein, N-His-tagged

• Cat.No.: ABL1-10M
• Product Overview: N-terminal 6Histagged, recombinant mouse Abl, amino acids 27–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The abl oncogene was initially identified as the viral transforming gene (v-abl) of Abelson murine leukemia virus (A-MuLV), from a chemically thymectomized mouse inoculated with Moloney murine leukemia virus (M-MuLV). The v-abl genome consists of M-MuLV gag sequences fused to mouse c-abl, resulting in the production of phosphoproteins, p160 and p120 gag/c-abl with protein-tyrosine kinase activity, as the major translational products. The abl oncogene is implicated in serval human leukemias including chronic myelocytic (CML), adult acute lymphoblastic (ALL) and pediatric ALL. In these leukemias the c-abl proto-oncogene undergoes a chromosomal translocation producing the Philadelphia (;) chromosome resulting in the fusion of c-abl sequences to the bcr gene of chromosome 22, and the molecular consequences of this translocation is the generation of a chimeric bcr/c-abl mRNA encoding activated abl protein-tyrosine kinase.
• Source: Sf21
• Species: Mouse
• Tag: His
• Protein length: 27-1123 aa
• Form: 3.19mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1 % 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1195U/mg, where one unit of Abl (mouse), active activity is defined as 1nmol phosphate incorporated into 50μM Abltide (EAIYAAPFAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 123.5 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPGE ALQRPVASDF EPQGLSEAAR WNSKENLLAG 61 PSENDPNLFV ALYDFVASGD NTLSITKGEK LRVLGYNHNG EWCEAQTKNG QGWVPSNYIT 121 PVNSLEKHSW YHGPVSRNAA EYLLSSGING SFLVRESESS PGQRSISLRY EGRVYHYRIN 181 TASDGKLYVS SESRFNTLAE LVHHHSTVAD GLITTLHYPA PKRNKPTIYG VSPNYDKWEM 241 ERTDITMKHK LGGGQYGEVY EGVWKKYSLT VAVKTLKEDT MEVEEFLKEA AVMKEIKHPN 301 LVQLLGVCTR EPPFYIITEF MTYGNLLDYL RECNRQEVSA VVLLYMATQI SSAMEYLEKK 361 NFIHRDLAAR NCLVGENHLV KVADFGLSRL MTGDTYTAHA GAKFPIKWTA PESLAYNKFS 421 IKSDVWAFGV LLWEIATYGM SPYPGIDLSQ VYELLEKDYR MERPEGCPEK VYELMRACWQ 481 WNPSDRPSFA EIHQAFETMF QESSISDEVE KELGKRGTRG GAGSMLQAPE LPTKTRTCRR 541 AAEQKDAPDT PELLHTKGLG ESDALDSEPA VSPLLPRKER GPPDGSLNED ERLLPRDRKT 601 NLFSALIKKK KKMAPTPPKR SSSFREMDGQ PDRRGASEDD SRELCNGPPA LTSDAAEPTK 661 SPKASNGAGV PNGAFREPGN SGFRSPHMWK KSSTLTGSRL AAAEEESGMS SSKRFLRSCS 721 ASCMPHGARD TEWRSVTLPR DLPSAGKQFD SSTFGGHKSE KPALPRKRTS ESRSEQVAKS 781 TAMPPPRLVK KNEEAAEEGF KDTESSPGSS PPSLTPKLLR RQVTASPSSG LSHKEEATKG 841 SASGMGTPAT AEPAPPSNKV GLSKASSEEM RVRRHKHSSE SPGRDKGRLA KLKPAPPPPP 901 ACTGKAGKPA QSPSQEAGEA GGPTKTKCTS LAMDAVNTDP TKAGPPGEGL RKPVPPSVPK 961 PQSTAKPPGT PTSPVSTPST APAPSPLAGD QQPSSAAFIP LISTRVSLRK TRQPPERIAS 1021 GTITKGVVLD STEALCLAIS RNSEQMASHS AVLEAGKNLY TFCVSYVDSI QQMRNKFAFR 1081 EAINKLESNL RELQICPATA SSGPAATQDF SKLLSSVKEI SDIVRR
• Purity: Purity 61% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Abl1 c-abl oncogene 1, non-receptor tyrosine kinase [ Mus musculus (house mouse) ]
• Official Symbol: ABL1
• Synonyms: ABL1; ABL proto-oncogene 1, non-receptor tyrosine kinase; Abl; c-Abl; E430008G22Rik
• Gene ID: 11350
• mRNA Refseq: NM_009594.4
• Protein Refseq: NP_033724.2
• UniProt ID: P00520