| Description : |
The mammalian Abelson (Abl) family of non-receptor tyrosine kinases is comprised of c-Abl (ABL1) and Arg (Abl-related gene, ABL2), which are encoded by the Abl1 and Abl2 genes, respectively. Deregulation of the c-Abl and Arg tyrosine kinases can occur as a result of chromosomal translocation events that produce chimeric proteins associated with the development of human leukemias as well as gastric cancer invasion and progression. GenBank XM_136360. The recombinant protein also contains 2 residues which are in conflict with GenBank XM_136360. These are M982V, reported in GenBank NM_005158, NM_007314, M35296, and an A inserted at position 562, reported in GenBank U40827. The residue coordinates in the native sequence have been given. |
| Source : |
Sf21 |
| Species : |
Mouse |
| Tag : |
His |
| Protein length : |
27-end |
| Form : |
3mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
2184U/mg, where one unit of Arg (mouse), active activity is defined as 1nmol phosphate incorporated into 50μM substrate peptide (EAIYAAPFAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
124.5 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GEVLHRPFGC DAESQALNEA IRWSSKENLL 61 GATESDPNLF VALYDFVASG DNTLSITKGE KLRVLGYNQN GEWSEVRSKN GQGWVPSNYI 121 TPVNSLEKHS WYHGPVSRSA AEYLLSSLIN GSFLVRESES SPGQLSISLR YEGRVYHYRI 181 NTTTDSKVYV TAESRFSTLA ELVHHHSTVA DGLVTTLHYP APKCNKPTVY GVSPIHDKWE 241 MERTDITMKH KLGGGQYGEV YVGVWKKYSL TVAVKTLKED TMEVEEFLKE AAVMKEIKHP 301 NLVQLLGVCT LEPPFYIVTE YMPYGNLLDY LRECSREEVT AVVLLYMATQ ISSAMEYLEK 361 KNFIHRDLAA RNCLVGENHV VKVADFGLSR LMTGDTYTAH AGAKFPIKWT APESLAYNTF 421 SIKSDVWAFG VLLWEIATYG MSPYPGIDLS QVYDLLEKGY RMEQPEGCPP KVYELMRACW 481 KWSPADRPSF AETHQAFETM FHDSSISEEV AEELGRTASS SSVVPYLPRL PLLPSKTRTL 541 RKQGENKENL DGGLDAAESL ASSSAPAGFI RSTQASSGSP ALPRKQRDKS PSSLLEDAKE 601 TCFTRDRKGG FFSSFMKKRN APTPPKRSSS FREMENQPHK KYELTGNFSP VASLQHADGF 661 SVAPSQQEPN LVPAKCYGGS FAQRNLCADD DSGGGGGSGT AGGGWSGITG FFTPRLIKKT 721 LGLRAGKPTA SDDTSKPFPR SNSTSSMSSG LPEQDRMAMT LPRNCQRSKL QLERTVSTSS 781 QPEENVDRAN DMLPKKSEEG AAPARERPKA KLLPRGATAL PLRAPDPAIT ESDSPGVGVA 841 GVAAAPKGKE RNGGTRLGVA GVPEDGEQLG WSSPAKAVAV LPTTHNHKVP VLISPTLKHT 901 PADVQLIGTD SQGNKFKLLS EHQVTSSGDK DRPRRVKPKC APPPPPVMRL LQHPSTCSDP 961 EEEPTAPPAG QHTPETQEGG KKAAPGPVPS SGKPGRPVMP PPQVPLPTSS ISPAKMANGT 1021 AGTKVALRKT KQAAEKISAD KISKEALLEC ADLLSSAITE PVPNSQLVDT GHQLLDYCSG 1081 YVDSIPQTRN KFAFREAVSK LELSLQELQV SSTAAGVPGT NPVLNNLLSC VQEISDVVQR |
| Purity : |
Purity 28% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
