Active Recombinant Rat ROCK1(2-543) Protein, N-His,N-HA-tagged

• Cat.No.: ROCK1-216H
• Product Overview: N-terminal 6His-tagged and N-terminal HA tagged, recombinant, rat ROKα/ROCKII, amino acids 2–543. Expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Rho-associated, coiled-coil containing protein kinase (ROCK), including the ROCK1 and ROCK2 isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. PKA and the two ROCKs have been reported to have opposite effects on neurite formation. More studies are needed to provide significant insights into the role of ROCK in several pathological processes. ROCK inhibitors have been shown the benefit for treatment of various cancers, inflammation, coronary artery spasms and hypertension.
• Source: Sf21
• Species: Human
• Tag: His,HA
• Protein length: 2-543 aa
• Form: 4.35mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1649U/mg, where one unit of ROKα/ROCKII activity is defined as 1nmol phosphate incorporated into 30μM long S6 substrate peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSEF ATMYPYDVPD YAPGAPEAAP GDGAGAGRQR 61 KLEALIRDPR SPINVESLLD GLNSLVLDLD FPALRKNKNI DNFLNRYEKI VKKIRGLQMK 121 AEDYDVVKVI GRGAFGEVQL VRHKASQKVY AMKLLSKFEM IKRSDSAFFW EERDIMAFAN 181 SPWVVQLFCA FQDDRYLYMV MEYMPGGDLV NLMSNYDVPE KWAKFYTAEV VLALDAIHSM 241 GLIHRDVKPD NMLLDKHGHL KLADFGTCMK MDETGMVHCD TAVGTPDYIS PEVLKSQGGD 301 GYYGRECDWW SVGVFLFEML VGDTPFYADS LVGTYSKIMD HKNSLCFPED TEISKHAKNL 361 ICAFLTDREV RLGRNGVEEI KQHPFFKNDQ WNWDNIRETA APVVPELSSD IDSSNFDDIE 421 DDKGDVETFP IPKAFVGNQL PFIGFTYFRE NLLLSDSPPC RENDAIQTRK SEESQEIQKK 481 LYALEEHLSS EVQAKEELEQ KCKSINTRLE KTAKELEEEI TFRKNVESTL RQLEREKALL 541 QHKNAEYQRK ADHEADKKRN LENDVNSLKD QLEDLKKRNQ SSQI
• Purity: Purity 63% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Rock1 Rho-associated coiled-coil containing protein kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: ROCK1
• Synonyms: ROCK1; Rho associated coiled-coil containing protein kinase 1; Rock-I; 1110055K06Rik
• Gene ID: 19877
• mRNA Refseq: NM_009071.2
• Protein Refseq: NP_033097.1
• UniProt ID: P70335