||Recombinant humanNPM1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||NPM1 is aphosphoprotein which moves between the nucleus and the cytoplasm. It isthought to be involved in several processes including regulation of theARF/p53 pathway. A number of genes are fusion partners have beencharacterized, in particular the anaplastic lymphoma kinase gene onchromosome 2. Mutations in NPM1 are associated with acute myeloid leukemia
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 10% glycerol 0.15M NaCl,1mM DTT
||34.7kDa (314aa),confirmed by MALDI-TOF
||> 85% by SDS -PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MEDSMDMDMS PLRPQNYLFG CELKADKDYH FKVDNDENEH QLSLRTVSLG AGAKDELHIVEAEAMNYEGSPIKVTLATLK MSVQPTVSLG GFEITPPVVL RLKCGSGPVH ISGQHLVAVE EDAESEDEEEEDVKLLSISG KRSAPGGGSK VPQKKVKLAADEDDDDDDEE DDDEDDDDDD FDDEEAEEKA PVKKSIRDTPAKNAQKSNQN GKDSKPSSTP RSKGQESFKK QEKTPKTPKG PSSVEDIKAKMQASIEKGGS LPKVEAKFINYVKNCFRMTD QEAIQDLWQW RKSL
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
||aurora B signaling; BARD1 signalingevents; chromosome Maintenance; deposition of New CENPA-containingNucleosomes at the Centromere; HIF-1-alpha transcription factor network; HIVInfection; host Interactions of HIV factors; interactions of Rev with hostcellular proteins; nuclear import of Rev protein; nucleosome assembly;validated targets of C-MYC transcriptional activation