Recombinant Human NAMPT protein(Met1-His491), His&GST-tagged

• Cat.No.: NAMPT-2531H
• Product Overview: Recombinant Human NAMPT (P43490) (Met 1-His 491) was expressed in Insect Cells, fused with a N-terminal polyhistidine tag followed by a GST tag.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-His491
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 0.3mM DTT Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human NAMPT/GST chimera consists of 728 amino acids and has a calculated molecular mass of 83.3 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens ]
• Official Symbol: NAMPT
• Synonyms: NAMPT; nicotinamide phosphoribosyltransferase; PBEF1, pre B cell colony enhancing factor 1; PBEF; visfatin; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; VF; PBEF1; VISFATIN; 1110035O14Rik; MGC117256; DKFZp666B131
• Gene ID: 10135
• mRNA Refseq: NM_005746
• Protein Refseq: NP_005737
• MIM: 608764
• UniProt ID: P43490

Reviews

06/24/2022

Does not trigger an immune response.

12/14/2021

Methods such as ELISA or immunostaining perform well.

11/13/2021

Easy to use, high stability, remarkable effect.

Q&As

What are the mutation types of NAMPT?

The types of mutations in NAMPT include point mutations, insertions/deletions, duplications, etc., which can cause structural and functional abnormalities of the protein.

How can diseases be treated by regulating NAMPT levels?

Some diseases related to NAD+ metabolism can be treated by regulating the level of NAMPT, such as by inhibiting its activity or regulating its expression.

How to study the regulatory mechanism of NAMPT?

Studying the regulatory mechanism of NAMPT requires a comprehensive use of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

What is the relationship of NAMPT to other genes or proteins?

NAMPT has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.

What is the specific role of NAMPT in cellular energy metabolism?

NAMPT plays an important role in cellular energy metabolism by catalyzing the synthesis of NAD+, an important intracellular coenzyme involved in various reactions of energy metabolism.

How are mutations in NAMPT detected and analyzed?

Mutations in NAMPT can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.