Recombinant Human Regulator Of Calcineurin 2, His-tagged

• Cat.No.: RCAN2-6859H
• Product Overview: Recombinant human RCAN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 24.1 kDa.

Specification

• Description: RCAN2 belonging to the RCAN (Regulator of calcineurin) family modulate the activity of Calcineurin through a valine-rich region within their carboxyl tail. RCANs compete with NFAT(nuclear factor of activated T-cells) for binding to the same docking site in calcineurin, which results in either inhibition or activation of calcineurin activity. RCAN2 is a Ca(2+) dependent protein phosphatase that is involed in several neuornal functions.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.2M NaCl.
• Molecular Mass: 24.1 kDa (217aa), confirmed by MALDI-TOF.
• Protein length: 1-197aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MPAPSMDCDV STLVACVVDV EVFTNQEVKE KFEGLFRTYD DCVTFQLFKS FRRVRINFSN PKSAARARIE LKLYFAQVQT PETDGDKLHL APPQPAKQFL ISPPSSPPVG WQPINDATPV LNYDLLYAVA KLGPGEKYEL HAGTESTPSV VVHVCDSDIE EEEDPKTSPK PKIIQTRRPG LPPSVSN
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: RCAN2 regulator of calcineurin 2 [ Homo sapiens ]
• Official Symbol: RCAN2
• Synonyms: RCAN2; regulator of calcineurin 2; Down syndrome critical region gene 1 like 1 , DSCR1L1; calcipressin-2; ZAKI 4; Down syndrome candidate region 1-like 1; thyroid hormone-responsive protein ZAKI-4; Down syndrome critical region gene 1-like 1; thyroid hormone-responsive (skin fibroblasts); myocyte-enriched calcineurin-interacting protein 2; CSP2; RCN2; MCIP2; ZAKI4; ZAKI-4; DSCR1L1
• Gene ID: 10231
• mRNA Refseq: NM_001251973
• Protein Refseq: NP_001238902
• MIM: 604876
• UniProt ID: Q14206
• Chromosome Location: 6p12.3
• Pathway: Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Role of Calcineurin-dependent NFAT signaling in lymphocytes, organism-specific biosystem
• Function: nucleotide binding; protein phosphatase 2B binding