"ROPN1" Related Products

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Recombinant Human ROPN1 293 Cell Lysate

Cat.No.: ROPN1-2251HCL
Description: Antigen standard for ropporin, rhophilin associated protein 1 (ROPN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ROPN1 rhophilin associated tail protein 1 [ Homo sapiens ]
Official Symbol: ROPN1
Synonyms: ROPN1; rhophilin associated tail protein 1; ropporin, rhophilin associated protein 1; ropporin-1A; cancer/testis antigen 91; CT91; ODF6; ROPN1A; ropporin; rhophilin-associated protein 1A; outer dense fiber of sperm tails 6; RHPNAP1; DKFZp434B1222;
Gene ID: 54763
mRNA Refseq: NM_017578
Protein Refseq: NP_060048
MIM: 611757
UniProt ID: Q9HAT0
Chromosome Location: 3q21.1
Function: cAMP-dependent protein kinase regulator activity;

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