Recombinant Zebrafish ANKLE2

• Cat.No.: ANKLE2-6712Z
• Product Overview: Recombinant Zebrafish ANKLE2 full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: ankle2 ankyrin repeat and LEM domain containing 2 [ Danio rerio (zebrafish) ]
• Official Symbol: ANKLE2
• Gene ID: 559297
• mRNA Refseq: NM_001126395
• Protein Refseq: NP_001119867
• UniProt ID: A2CEB2

Reviews

04/25/2020

This protein demonstrates exceptional performance in ELISA, delivering accurate and reliable results in the detection and quantification of specific antigens.

12/18/2018

ANKLE2 protein has proven to be instrumental in protein electron microscopy structure analysis.

01/14/2018

ANKLE2 protein is highly recommended for scientific research applications, especially in ELISA assays and protein electron microscopy structure analysis.

11/07/2016

With ANKLE2 protein, researchers can visualize protein complexes and their interactions with remarkable clarity, unraveling important insights into their functional mechanisms.

Q&As

Which tissues or organs express the ANKLE2 protein?

The ANKLE2 protein is expressed in various tissues and organs, including the brain, liver, heart, kidney, and skeletal muscles. Its expression is particularly high in tissues with a high turnover rate, such as developing embryos and rapidly dividing cell types.

Can ANKLE2 mutations be inherited?

ANKLE2 mutations associated with disorders like IMAGe syndrome can be inherited in an autosomal dominant manner. This means that an affected individual has a 50% chance of passing the mutation onto their offspring. However, it is important to note that ANKLE2-related disorders are rare and mutations in the ANKLE2 gene are not commonly found in the general population. Genetic testing and counseling can help assess the risk of inheritance and provide information for family planning.

What other functions or pathways might ANKLE2 be involved in?

Besides its known roles in nuclear envelope organization and DNA repair, ANKLE2 has been implicated in other cellular processes. It has been shown to participate in cell cycle regulation by influencing the expression of cyclin-dependent kinase (CDK) inhibitors. ANKLE2 has also been found to play a role in the DNA damage response pathway, specifically in the activation of checkpoint kinases. Further research is needed to fully understand the extent of ANKLE2's involvement in these processes.

What are the potential implications of ANKLE2 protein research for therapeutics?

Given the critical roles of ANKLE2 in nuclear envelope function, DNA repair, and chromosome segregation, understanding its involvement in disease processes may have therapeutic implications. Dysregulation of ANKLE2 has been linked to certain developmental disorders and cancer progression. Targeting ANKLE2 or its interacting partners could potentially lead to novel strategies for treating these diseases. However, more research is necessary to fully explore the therapeutic potential of ANKLE2 protein.

Are there any animal models or experimental systems used to study the ANKLE2 protein?

Animal models, such as mice, have been used to study the function of ANKLE2 in development and disease. Genetic modifications that disrupt or alter ANKLE2 expression in these models can provide insights into its role in different biological processes. Cell culture systems and molecular techniques are also employed to investigate the molecular mechanisms and interactions of ANKLE2.

Are there any targeted therapies or treatment options available for ANKLE2-related disorders?

Currently, there are no targeted therapies available specifically for ANKLE2-related disorders. Treatment for these disorders is generally symptomatic and supportive, aiming to address the specific symptoms and complications that arise. For example, individuals with IMAGe syndrome may receive hormone replacement therapy to manage adrenal insufficiency, while skeletal abnormalities may require orthopedic interventions. Genetic counseling and early detection of potential complications are important components of managing ANKLE2-related disorders.

How is the ANKLE2 protein regulated within cells?

The regulation of ANKLE2 protein is not well understood. It is known to have multiple isoforms, which may be differentially regulated. Additionally, post-translational modifications and interacting proteins may influence its stability, localization, and activity. Further research is needed to uncover the specific regulatory mechanisms of ANKLE2.

Are there any known interacting partners of the ANKLE2 protein?

Yes, ANKLE2 has been found to interact with various proteins and complexes. One well-characterized interaction is with the barrier-to-autointegration factor (BAF) protein, which is involved in nuclear envelope assembly and maintenance. ANKLE2 interacts with BAF to regulate nuclear envelope remodeling and chromatin organization. Additionally, ANKLE2 has been shown to interact with proteins involved in DNA repair, such as BRCA1 and Rad50, indicating its role in these cellular processes.

Are there any clinical trials or ongoing research focused on ANKLE2-related disorders?

Due to the rarity of ANKLE2-related disorders, clinical trials and research specifically focused on these disorders are limited. However, research is ongoing to better understand the molecular mechanisms and clinical implications of ANKLE2 mutations. Additionally, studies investigating the function of ANKLE2 in cancer and other cellular processes may provide valuable insights into ANKLE2-related disorders. It is important to stay updated on current literature and research to keep track of any advancements in this field.

Are there any known mutations in the ANKLE2 gene associated with disease?

Yes, mutations in the ANKLE2 gene have been associated with IMAGe syndrome, as mentioned earlier. These mutations can disrupt the function of ANKLE2 and lead to the characteristic symptoms of the disorder. However, the exact mechanisms by which these mutations result in IMAGe syndrome are still being investigated. Other disease-associated mutations or dysregulation of ANKLE2 are yet to be identified and characterized.

Is ANKLE2 protein implicated in cancer?

Yes, there is evidence suggesting the involvement of ANKLE2 in cancer. Studies have found that ANKLE2 expression is elevated in certain types of cancer, including ovarian cancer, lung cancer, and pancreatic cancer. Elevated ANKLE2 expression has been associated with tumor progression and poor patient survival outcomes. The exact mechanisms through which ANKLE2 contributes to cancer development and progression are still being investigated.

Are there any animal models available for studying ANKLE2-related disorders?

As of now, there are no specific animal models available for studying ANKLE2-related disorders such as IMAGe syndrome. This lack of animal models makes it challenging to fully understand the molecular mechanisms and pathways affected by ANKLE2 mutations. However, ongoing research efforts may lead to the development of animal models in the future, which would greatly facilitate the study of ANKLE2-related disorders and potential therapeutic interventions.

What are the developmental disorders associated with dysregulation of ANKLE2?

Mutations in the ANKLE2 gene have been identified in individuals with a condition called IMAGe syndrome, which stands for Intrauterine growth restriction, Metaphyseal dysplasia, Adrenal hypoplasia congenita, and Genital anomalies. IMAGe syndrome is a rare disorder characterized by prenatal growth restriction, skeletal abnormalities, adrenal gland underdevelopment, and genital anomalies. ANKLE2 mutations are thought to disrupt the function of the protein, leading to the development of these symptoms.