||Recombinant human macrophage migration inhibitory factor (glycosylation-inhibiting factor), fused to His-tag at N-terminus, was cloned into anE. coliexpression vector and was purified to apparent homogeneity by using conventional column chromatography techniques. Macrophage Inducing Factor is a single, non-glycosylated, polypeptide chain containing amino acids from 1-114 and having a molecular mass of 16.6 kDa.
||The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
||1mg/ml solution containing PBS pH-7.4.
|Amino Acid Sequence:
||MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||Liquid MIF although stable 14℃ for 1 week, should be stored desiccated below -18℃. Please prevent freeze-thaw cycles.