Recombinant Human SERPINA12 protein

• Cat.No.: SERPINA12-987H
• Product Overview: Recombinant Human SERPINA12 protein was expressed in Escherichia coli.

Specification

• Source: E.coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20.
• Molecular Mass: Approximately 45.1 kDa, a single non-glycosylated polypeptide chain containing 394 amino acids.
• Protein length: 394
• AA Sequence: LKPSFSPRNYKALSEVQGWKQRMAA KELARQNMDLGFKLLKKLAFYNPGR NIFLSPLSISTAFSMLCLGAQDSTL DEIKQGFNFRKMPEKDLHEGFHYII HELTQKTQDLKLSIGNTLFIDQRLQ PQRKFLEDAKNFYSAETILTNFQNL EMAQKQINDFISQKTHGKINNLIEN IDPGTVMLLANYIFFRARWKHEFDP NVTKEEDFFLEKNSSVKVPMMFRSG IYQVGYDDKLSCTILEIPYQKNITA IFILPDEGKLKHLEKGLQVDTFSRW KTLLSRRVVDVSVPRLHMTGTFDLK KTLSYIGVSKIFEEHGDLTKIAPHR SLKVGEAVHKAELKMDERGTEGAAG TGAQTLPMETPLVVKIDKPYLLLIY SEKIPSVLFLGKIVNPIGK
• Endotoxin: Less than 0.1 EU/μg of rHuVaspin as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: SERPINA12
• Official Symbol: SERPINA12
• Synonyms: SERPINA12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serine (or cysteine) proteinase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 12; serpin A12; OL 64; Vaspin; vaspin; visceral adipose-specific SERPIN; visceral adipose tissue-derived serine protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; OL-64
• Gene ID: 145264
• mRNA Refseq: NM_173850
• Protein Refseq: NP_776249
• MIM: 617471
• UniProt ID: Q8IW75