||Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine. TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis and platelet production.
||Sf 21 Insect Cells.
||The predicted molecular weight of Recombinant Human TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 47 kDa.
|State Of Matter:
||The biological activity of Human Thrombopoietin is determined by the dose-dependent stimulation of the proliferation of human MO7e cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
||>97% by SDS Page and analyzed by silver stain.
||<1.0 EU/µg as determined by the LAL method.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.