Active GMP Recombinant Human NRG1 protein

• Cat.No.: NRG1-140HG
• Product Overview: GMP Recombinant Human NRG1 protein(61 aa) was expressed in E. coli in an animal component free process under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 5 U/mg.
• Molecular Mass: Approximately 7.0 kDa, a single non-glycosylated polypeptide chain containing 61 amino acids.
• AA Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKAEELY Q
• Endotoxin: Less than 0.01 EU/μg of rHuNRG1-β2 GMP as determined by LAL method.
• Purity: > 98 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  - 12 months from date of receipt, -20 to -70 °C as supplied.
  - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homo sapiens ]
• Official Symbol: NRG1
• Synonyms: NRG1; neuregulin 1; HGL; pro-neuregulin-1, membrane-bound isoform; GGF; HRG; NDF; MSTP131; pro-NRG1; glial growth factor; neu differentiation factor; neuregulin 1 type IV beta 3; neuregulin 1 type IV beta 1a; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); ARIA; GGF2; HRG1; HRGA; SMDF; MST131
• Gene ID: 3084
• mRNA Refseq: NM_001159995
• Protein Refseq: NP_001153467
• MIM: 142445
• UniProt ID: Q02297