Recombinant Human IL27B & IL12A protein(Met1-Lys229 & Met1-Ser219), Flag&His-tagged

• Cat.No.: IL27B & IL12A-1264H
• Product Overview: Recombinant Human IL27B (NP_005746.2)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant Human IL12A (P29459-1)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human IL27B&IL12A heterodimer was purified and expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: C-Flag&His
• Protein length: Met1-Lys229 & Met1-Ser219
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of Human IL27B&IL12A comprises 425 (217+208) amino acids and has a calculated molecular mass of 48.3(24.3+24) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Official Symbol: IL27B; IL12A

Gene Information

• Official Symbol: IL27B; IL12A