Recombinant Human IL7RA & IL2RG protein(Met1-Asp239 & Met1-Asn254), His-tagged
Cat.No. : | IL7RA & IL2RG-3390H |
Product Overview : | Recombinant Human IL7RA (P16871-1)(Met1-Asp239) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Human IL2RG (P31785-1) (Met1-Asn254) was expressed in HEK293 with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL7RA & IL2RG heterodimer was purified. |
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Source : | HEK293 |
Species : | Human |
Tag : | C-His |
Protein length : | Met1-Asp239 & Met1-Asn254 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant heterodimer of human IL7RA & IL2RG comprises 473 (230+243) amino acids and has a calculated molecular mass of 55.54 (26.71+28.83) KDa. The apparent molecular mass of human IL7RA & IL2RG heterodimer is approximately 49.70 KDa respectively in SDS-PAGE. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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