Recombinant Human KDM6B(1141-1641) Protein

• Cat.No.: KDM6B-120H
• Product Overview: Recombinant, amino acids 1141-1641, human JMJD3, expressed in E.coli. Purified using immobilised metal affinity chromatography.

Specification

• Description: JMJD3 (Jumonji domain-containing protein 3), also known as KDM6B, is a histone demethylase that belongs to the JmjC domain-containing protein family. JMJD3 specifically targets tri-methylated lysine 27 on histone H3 (H3K27me3), and catalyzes its removal, leading to changes in chromatin structure and gene expression. JMJD3 is expressed in a variety of tissues and is involved in the regulation of various cellular processes, including differentiation, proliferation, and apoptosis. JMJD3 has been identified as a potential therapeutic target for various diseases, including cancer, inflammation, and cardiovascular diseases. Its inhibition has been shown to suppress tumor growth and improve response to chemotherapy in various types of cancer. Therefore, JMJD3 is an important epigenetic protein with potential therapeutic applications.
• Source: E. coli
• Species: Human
• Protein length: 1141-1641 aa
• Form: 1mg/ml of enzyme in 25mM Hepes/NaOH pH 7.5, 250mM NaCl, 50% glycerol. Frozen solution.
• Molecular Mass: 57.2 kDa
• AA Sequence: 1 SMDVVRASRN AKVKGKFRES YLSPAQSVKP KINTEEKLPR EKLNPPTPSI 51 YLESKRDAFS PVLLQFCTDP RNPITVIRGL AGSLRLNLGL FSTKTLVEAS 101 GEHTVEVRTQ VQQPSDENWD LTGTRQIWPC ESSRSHTTIA KYAQYQASSF 151 QESLQEEKES EDEESEEPDS TTGTPPSSAP DPKNHHIIKF GTNIDLSDAK 201 RWKPQLQELL KLPAFMRVTS TGNMLSHVGH TILGMNTVQL YMKVPGSRTP 251 GHQENNNFCS VNINIGPGDC EWFAVHEHYW ETISAFCDRH GVDYLTGSWW 301 PILDDLYASN IPVYRFVQRP GDLVWINAGT VHWVQATGWC NNIAWNVGPL 351 TAYQYQLALE RYEWNEVKNV KSIVPMIHVS WNVARTVKIS DPDLFKMIKF 401 CLLQSMKHCQ VQRESLVRAG KKIAYQGRVK DEPAYYCNEC DVEVFNILFV 451 TSENGSRNTY LVHCEGCARR RSAGLQGVVV LEQYRTEELA QAYDAFTLVR 501 ARR
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: Stable for 1 year at -70 centigrade from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: KDM6B lysine demethylase 6B [ Homo sapiens (human) ]
• Official Symbol: KDM6B
• Synonyms: KDM6B; lysine demethylase 6B; JMJD3; NEDCFSA
• Gene ID: 23135
• mRNA Refseq: NM_001080424.2
• Protein Refseq: NP_001073893.1
• MIM: 611577
• UniProt ID: O15054