Active Recombinant Human NOG Protein, Pre-aliquoted

• Cat.No.: NOG-29H
• Product Overview: Recombinant Human NOG Protein, Gln28 - Cys232, was expressed in Mouse myeloma cell line.

Specification

• Description: The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.
• Source: Mouse myeloma cell line
• Species: Human
• Bio-activity: Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.
• Molecular Mass: Predicted Molecular Mass:23 kDa (monomer)
  SDS-PAGE: 30-33 kDa, reducing conditions
• Protein length: Gln28-Cys232
• N-terminal Sequence Analysis: No results obtained: Gln28 predicted
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 250 μg/mL in sterile PBS. For immediate use, simply roll Pre-aliquoted directly into cell culture medium.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: NOG noggin [ Homo sapiens (human) ]
• Official Symbol: NOG
• Synonyms: NOG; noggin; SYM1; SYNS1; SYNS1A; noggin; symphalangism 1 (proximal)
• Gene ID: 9241
• mRNA Refseq: NM_005450
• Protein Refseq: NP_005441
• MIM: 602991
• UniProt ID: Q13253