NOG

  • Official Full Name

    noggin

  • Overview

    The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse.
  • Synonyms

    NOG; noggin; SYM1; SYNS1;

  • Recombinant Proteins
  • GMP Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Dog
  • Human
  • Mouse
  • Mouse/Rat
  • Pig
  • Rat
  • Rhesus Macaque
  • CHO
  • CHO-K1
  • E. coli
  • E.coli
  • HEK293
  • Human Cell
  • Human cells
  • In Vitro Cell Free System
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Nicotiana Benthamiana
  • NS0
  • Wheat Germ
  • C
  • Fc
  • hFc
  • His
  • GST
  • His (Fc)
  • Avi
  • His|GST
  • His|MBP
  • Myc
  • human|IgG1|Fc
  • N/A
  • N
  • MBP
  • Strep II
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human NOG-2745H Active Recombinant Human Noggin Nicotiana Benthamiana N/A
Human NOG-204H Active Recombinant Human NOG protein HEK293 N/A 205
Human NOG-01HG Active GMP Recombinant Human NOG Protein CHO-K1
Human NOG-066N Active Recombinant Human NOG Protein (206 aa) E.coli 206
Human NOG-001H Active Recombinant Human NOG Protein HEK293
Human NOG-1993H Recombinant Human NOG protein, His-tagged, Animal-Free E. coli C-His
Human NOG-119HG Active GMP Recombinant Human NOG protein HEK293 DNA sequence encoding Human noggin(NP_005441.1) was expressed with His tag at the C-terminal.
Human NOG-817H Recombinant Human NOG protein(Met1-Cys232), hFc-tagged HEK293 C-hFc Met1-Cys232
Human NOG-223H Recombinant Human NOG, StrepII-tagged Human Cell Strep II 28-232, 205 a.a.
Human NOG-046H Recombinant Human NOG protein, Fc-tagged HEK293 human/IgG1/Fc Gln28-Cys232
Human NOG-3156H Active Recombinant Human NOG protein(Met1-Cys232), His-tagged HEK293 C-His Met1-Cys232
Human NOG-30336TH Recombinant Human NOG E.coli N/A
Human NOG-1323H Recombinant Human NOG, His-tagged E.coli His 1-232aa
Human NOG-30339TH Recombinant Human NOG N/A
Human NOG-21H Recombinant Human NOG protein E.coli N/A 206
Human NOG-454H Recombinant Human Noggin HEK293 N/A
Human NOG-2414HCL Recombinant Human NOG cell lysate Human Cell N/A
Human NOG-120HG Active GMP Recombinant Human NOG Protein Mammalian cells N/A
Human NOG-117HG Active GMP Recombinant Human NOG Protein Mammalian cells Fc
Human NOG-27H Recombinant Human NOG Protein E.coli
Human NOG-5966H Recombinant Human NOG Protein, GST-tagged Wheat Germ GST
Human NOG-293H Recombinant Human NOG protein Insect Cell N/A 205
Human NOG-03HG Active Recombinant Human NOG Protein (Gln28-Cys232), C-Fc-tagged, GMP Grade NS0 C-Fc Gln28-Cys232
Human NOG-29H Active Recombinant Human NOG Protein, Pre-aliquoted NS0 Gln28-Cys232
Human NOG-42H Active Recombinant Human NOG Protein, Animal Free Human cells
Human NOG-213N Active Recombinant Human NOG Protein CHO
Human NOG-6681HF Recombinant Full Length Human NOG Protein, GST-tagged In Vitro Cell Free System GST 232 amino acids
Human NOG-1423H Recombinant Human NOG protein, His-GST & Myc-tagged E.coli His-GST & Myc 28-232aa
Human NOG-126H Recombinant Human NOG Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human NOG-2849H Recombinant Human NOG protein(28-232 aa), N-MBP & C-His-tagged HEK293 N-MBP & C-His 28-232 aa
Human NOG-126H-B Recombinant Human NOG Protein Pre-coupled Magnetic Beads HEK293
Human NOG-2850H Recombinant Human NOG protein(28-232 aa), N-MBP & C-His-tagged HEK293 N-MBP & C-His 28-232 aa
Human NOG-401H Recombinant Human NOG Protein, Fc-tagged HEK293 Fc 232
Human NOG-3367H Recombinant Human NOG Protein (Gln28-Cys232), His tagged E.coli His Gln28-Cys232
Human NOG-315H Active Recombinant Human NOG Protein (Gln28-Cys232), C-Fc tagged, Animal-free, Carrier-free HEK293 C-Fc Gln28-Cys232
Human NOG-321H Recombinant Human NOG Protein, His-tagged E.coli His Gln28~Cys232
Mouse NOG-887M Active Recombinant Mouse NOG protein(Met1-Cys232), hFc-tagged HEK293 C-hFc Met1-Cys232
Mouse Nog-4452M Active Recombinant Mouse Nog Protein E.coli
Mouse Nog-019N Active Recombinant Mouse Nog Protein (206 aa) E.coli 206
Mouse Nog-954M Recombinant Mouse Nog protein E.coli N/A 206
Mouse Nog-1770M Recombinant Mouse Noggin Mammalian cells N/A
Mouse Nog-10603M Recombinant Mouse Nog Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Nog-10603M-B Recombinant Mouse Nog Protein Pre-coupled Magnetic Beads HEK293
Mouse Nog-322M Recombinant Mouse Nog Protein, His/GST-tagged E.coli His/GST Met116~Cys232
Mouse Nog-212N Active Recombinant Mouse Nog Protein CHO
Rat Nog-590R Recombinant Rat Nog protein, His & MBP-tagged E.coli His/MBP Met28~Cys144
Rat Nog-324R Recombinant Rat Nog Protein, His/GST-tagged E.coli His/GST Gly1~Trp117
Rat Nog-1834R Recombinant Rat Nog Protein, His&GST-tagged E.coli N-His&GST Met28-Cys144
Rat Nog-325R Recombinant Rat Nog Protein, His/MBP-tagged E.coli His/MBP Met28~Cys144
Dog NOG-319D Recombinant Dog NOG Protein, His-tagged E.coli His Gln28~Cys240
Mouse/Rat Nog-28M Recombinant Mouse/Rat Nog Protein E.coli
Rhesus Macaque NOG-3062R Recombinant Rhesus monkey NOG Protein, His-tagged Mammalian Cell His
Rhesus Macaque NOG-2881R Recombinant Rhesus Macaque NOG Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque NOG-2881R-B Recombinant Rhesus Macaque NOG Protein Pre-coupled Magnetic Beads HEK293
Pig NOG323P Recombinant Pig NOG Protein, His-tagged E.coli His Gln28~Cys232
Chicken NOG-5699C Recombinant Chicken NOG Mammalian Cell His
Chicken NOG-320C Recombinant Chicken NOG Protein, His-tagged E.coli His Gln27~Cys223
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is NOG protein?

NOG (noggin) gene is a protein coding gene which situated on the long arm of chromosome 17 at locus 17q22. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. The NOG protein is consisted of 232 amino acids and its molecular mass is approximately 25.8 kDa.

What is the function of NOG protein?

NOG is an antagonist of bone morphogenetic proteins (BMPs), which are part of the transforming growth factor-beta (TGF-β) superfamily. The primary function of NOG protein is to bind and inactivate BMPs, thereby modulating the BMP signaling pathway. In the context of bone and cartilage formation, NOG protein plays a significant role. It can effectively attenuate osteoarthritis (OA) by protecting cartilage and preventing its degradation, as indicated by research that demonstrates the therapeutic potential of NOG protein in treating OA. Furthermore, NOG protein is involved in numerous developmental processes such as neural tube fusion and joint formation. In addition to its role in development and bone metabolism, NOG protein is also used in the field of organoid culture. It is added to the culture medium to coordinate Wnt signal activation in stem cells, promoting their proliferation.

NOG Related Signaling Pathway

NOG can bind and inhibit the binding of Wnt protein to LRP5/6 receptor, thereby preventing Wnt protein from entering the cell and further inhibiting the activation of β-catenin, thereby inhibiting the Wnt/β-catenin signaling pathway. NOG can bind and inhibit the binding of BMP protein to its receptor, thereby preventing BMP protein from entering the cell and further inhibiting the activation of Smad protein. NOG can bind and activate the FGF receptor, thereby further activating the FGF signaling pathway and promoting cell proliferation and differentiation. NOG can bind and activate TGF-β receptors, thereby further activating the TGF-β signaling pathway and promoting cell proliferation and differentiation.

BMP signalling.jpg

Fig1. BMP signalling. (Bo Kyoung Kang, 2023)

NOG Related Diseases

NOG plays an important role in nervous system development, and its abnormal expression is associated with neural tube defects, hydrocephalus, autism and other neurological developmental disorders. NOG is also involved in the regulation of bone development, and its abnormal expression is associated with spina bifida, polydactyly, osteoporosis and other skeletal abnormalities. The abnormal expression of NOG is associated with the occurrence and development of various tumors, such as breast cancer, gastric cancer, and lung cancer. NOG is also associated with the occurrence and development of some other diseases, such as cardiovascular disease, diabetes, liver disease, etc.

Bioapplications of NOG

Tetrahydropyrimidine (Ectoine) is a cyclic amino acid derived from L-aspartic acid that acts as a protective and stabilizing agent and plays an important role in cells in response to various external pressures, such as high temperature, high osmotic pressure, drying, and radiation. NOG protein has made important progress in the efficient preparation of tetrahydropyrimidine, and through the strategy of combining metabolic engineering and enzyme engineering, it has achieved efficient synthesis in Escherichia coli.

Due to the protective and stabilizing effects of tetrahydropyrimidine, NOG proteins also have applications in the cosmetic industry, especially in the development of products that protect the skin from environmental stress.

Studies have shown that NOG proteins can effectively reduce the condition of osteoarthritis (OA) by protecting the cartilage and preventing its degeneration, which indicates that NOG proteins have potential applications in the treatment of cartilage degenerative diseases such as OA.

High Purity

SDS-PAGE (NOG-204H).jpg

Fig1. SDS-PAGE (NOG-204H)

High Bioactivity

Activity Data (NOG-204H).jpg

Fig2. Activity Data (NOG-204H)

Case Study 1: Karolina Truchan, 2024

The balance between Noggin and bone morphogenetic proteins (BMPs) is important during early development and skeletal regenerative therapies. Noggin binds BMPs in the extracellular space, thereby preventing BMP signaling. However, Noggin may affect cell response not necessarily through the modulation of BMP signaling, raising the possibility of direct Noggin signaling through yet unspecified receptors. Here we show that in osteogenic cultures of adipose-derived stem cells (ASCs), Noggin activates fibroblast growth factor receptors (FGFRs), Src/Akt and ERK kinases, and it stabilizes TAZ proteins in the presence of dexamethasone. Overall, this leads ASCs to increased expression of osteogenic markers and robust mineral deposition. The results also indicate that Noggin can induce osteogenic genes expression in normal human bone marrow stem cells and alkaline phosphatase activity in normal human dental pulp stem cells. Besides, Noggin can specifically activate FGFR2 in osteosarcoma cells.

Alkaline phosphatase (ALP) activity.jpg

Fig1. Alkaline phosphatase (ALP) activity after 7-d culture of normal human bone marrow stromal cells.

Phosphorylation of FGFR Type 2 (p-FGFR2).jpg

Fig2. Phosphorylation of FGFR Type 2 (p-FGFR2) after 1-h Noggin stimulation of SaOS-2 cells, that are abundant in FGFRs.

Case Study 2: Chiara Secondini, 2011

Members of the BMP and Wnt protein families play a relevant role in physiologic and pathologic bone turnover. Extracellular antagonists are crucial for the modulation of their activity. Lack of expression of the BMP antagonist noggin by osteoinductive, carcinoma-derived cell lines is a determinant of the osteoblast response induced by their bone metastases. In contrast, osteolytic, carcinoma-derived cell lines express noggin constitutively. Intra-osseous xenografts of PC-3 prostate cancer cells induced osteolytic lesions characterized not only by enhanced osteoclast-mediated bone resorption, but also by decreased osteoblast-mediated bone formation. Bone formation was preserved in the osteolytic lesions induced by noggin-silenced PC-3 cells, suggesting that cancer cell-derived noggin interferes with physiologic bone coupling. Furthermore, intra-osseous tumor growth of noggin-silenced PC-3 cells was limited, most probably as a result of the persisting osteoblast activity. This investigation provides new evidence for a model of osteolytic bone metastasis where constitutive secretion of noggin by cancer cells mediates inhibition of bone formation, thereby preventing repair of osteolytic lesions generated by an excess of osteoclast-mediated bone resorption.

Noggin protein secreted in the conditioned medium (CM).jpg

Fig3. Noggin protein secreted in the conditioned medium (CM) from PC-3/Fluc, mock and Nog-KD clones.

Noggin silencing limits growth.jpg

Fig4. Noggin silencing limits growth of PC-3/Fluc cells in bone xenografts.

NOG involved in several pathways and played different roles in them. We selected most pathways NOG participated on our site, such as BMP receptor signaling, BMP signalling and regulation, Cardiac Progenitor Differentiation, which may be useful for your reference. Also, other proteins which involved in the same pathway with NOG were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
BMP receptor signalingFST;CTDSP2;CALML4;RGMB;RGMA;CHRDL1;SOSTDC1;SKI;NOG
BMP signalling and regulationNOG;TOB1
Cardiac Progenitor DifferentiationTBX5;IRX4;SOX1;ZFP42;NKX2;SIRPA;LIN28B;BMP1;T
Signal TransductionDRAP1;UTS2D;NRP2;SEPT7;ARHGAP4;METAP2A;NPFF;CLIP3;GPR68
Signaling by BMPFSTL1;NOG;SKIB;GREM2;CALML4;FSTL1A;SKIA;NOG1;CHRDL1
TGF Beta Signaling PathwayENG;ZFP423;TGFBR3;ZNF423;FST;FOXH1;RUNX3;LEFTY1;NOG
TGF-beta signaling pathwayRPS6KB1;TFDP1;BMP4;ID2A;TGFBR2;BMP2B;PPP2R1A;CUL1;ACVR1C

NOG has several biochemical functions, for example, cytokine binding, protein binding, protein complex binding. Some of the functions are cooperated with other proteins, some of the functions could acted by NOG itself. We selected most functions NOG had, and list some proteins which have the same functions with NOG. You can find most of the proteins on our site.

Function Related Protein
cytokine bindingTNFRSF11A;NRP1;IL31RA;CXCR4;PARK7;KITA;CRLF1;IL17F;CD74
protein bindingMARCH10;CDK20;TUBB2B;CXCR2;GUCY1A3;PXMP2;LSM6;UBE2I;SLAMF7
protein complex bindingCRTAP;LCA5;AP2B1;CYFIP1;CRHR1;STRN4;GNB4;RAN;CRIPT
protein homodimerization activityVAPB;CARS;RABEP1;NPR3;GABRR1;EXT1;APOE;TCF12;CHMP4B

NOG has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NOG here. Most of them are supplied by our site. Hope this information will be useful for your research of NOG.

BMP2; BMP7; GDF5

Research Area

Related articles

Ohshima, S; Mori, S; et al. Differentiation ability of multipotent hematopoietic stem/progenitor cells detected by a porcine specific anti-CD117 monoclonal antibody. BIOSCIENCE TRENDS 8:308-315(2014).
Ishikawa, Y; Usui, T; et al. Functional engraftment of human peripheral T and B cells and sustained production of autoantibodies in NOD/LtSzscid/IL-2R gamma(-/-) mice. EUROPEAN JOURNAL OF IMMUNOLOGY 44:3453-3463(2014).
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Customer Reviews (3)

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Reviews
07/15/2022

    Efficient peptide synthesis, accelerates our peptide research projects.

    04/30/2018

      Fast and reliable protein expression, aids our experiments significantly.

      01/25/2018

        Dependable antibody validation, crucial for our immunoprecipitation studies.

        Q&As (7)

        Ask a question
        How does NOG (Noggin) modulate bone morphogenetic protein (BMP) signaling in development and tissue repair? 10/25/2022

        NOG inhibits BMP signaling, critical for tissue development and repair processes.

        What are the mechanisms by which NOG modulates embryonic patterning and organogenesis? 09/05/2021

        NOG modulates embryonic patterning and organogenesis, guiding proper organ development.

        How do alterations in NOG expression or function affect the development of congenital anomalies? 02/08/2020

        Changes in NOG expression or function can lead to congenital anomalies due to disrupted developmental processes.

        How does NOG contribute to the regulation of stem cell differentiation? 07/11/2019

        NOG regulates stem cell differentiation, influencing their development into various tissue types.

        What role does NOG play in skeletal development and the regulation of bone growth? 02/11/2019

        NOG plays a key role in skeletal development by regulating bone growth and differentiation.

        How does NOG influence neural development and neurogenesis? 02/03/2019

        NOG influences neural development, impacting the formation and specialization of neural cells.

        What is the impact of NOG in joint and cartilage health, particularly in arthritis? 01/07/2018

        NOG is important for joint and cartilage health, potentially impacting arthritis progression.

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