Recombinant Human PLOD1 protein, MYC/DDK-tagged

• Cat.No.: PLOD1-152H
• Product Overview: Recombinant Human PLOD1 fused with MYC/DDK tag at C-terminal was expressed in HEK293.

Specification

• Description: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 81.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: PLOD1 procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 [ Homo sapiens ]
• Official Symbol: PLOD1
• Synonyms: LH; LH1; LLH; EDS6; PLOD; lysine hydroxylase; lysyl hydroxlase 1; procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
• Gene ID: 5351
• mRNA Refseq: NM_000302
• Protein Refseq: NP_000293
• MIM: 153454
• UniProt ID: Q02809
• Chromosome Location: 1p36.22
• Pathway: Collagen biosynthesis and modifying enzymes, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Lysine degradation, conserved biosystem
• Function: iron ion binding; protein homodimerization activity; procollagen-lysine 5-dioxygenase activity