Recombinant Human SELE Protein, His-tagged

• Cat.No.: SELE-215H
• Product Overview: Biotinylated Recombinant Human E-selectin is produced by Human 293 Cells. The target gene encoding W22-P556 is expressed with an 8His tag at the C terminus.

Specification

• Description: E-Selectin is a kDa cell surface glycoprotein that belongs to the selectin family. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, E-selectin plays an important role in recruiting leukocytes to the site of injury during inflammation. E-selectin also mediates the adhesion of tumor cells to endothelial cells, through binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells. In addition, studies have found that levels of E-Selectin may be elevated in subjects with various pathological conditions.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized frum a 0.2 um filtered solution of PBS, pH7.2.
• Molecular Mass: Predicted Molecular Weight: 61.9kDa
  Apparent Molecular Weight: Biotinylated E-selectin appeared as a smear between 66.2-116kDa in a reducing SDS-PAGE gel due to glycosylation.
• AA Sequence: W22-P556
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: greater than 95% as determined by reducing SDS-PAGE.
• Shipping: The product is shipped on dry ice pack.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: SELE selectin E [ Homo sapiens (human) ]
• Official Symbol: SELE
• Synonyms: E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; ELAM; ESEL; CD62E; ELAM1; LECAM2; selectin-e
• Gene ID: 6401
• mRNA Refseq: NM_000450.2
• Protein Refseq: NP_000441.2
• MIM: 131210
• UniProt ID: P16581