Recombinant Mouse IL15 & IL15RA protein(Met1-Ser162 & Met1-Lys205), His-tagged

• Cat.No.: IL15 & IL15RA-1159M
• Product Overview: Recombinant Mouse IL15 (P48346-1)(Met1-Ser162) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse IL15RA (Q60819-1) (Met1-Lys205) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL15 & IL15RA heterodimer was purified in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ser162 & Met1-Lys205
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of mouse IL15 & IL15RA comprises 308 (125+183) amino acids and has a calculated molecular mass of 34.46 (14.70+19.76) kDa. The apparent molecular mass of mouse IL15 & IL15RA heterodimer is approximately (50.02, 23.49, 21.78 and 19.76) kDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il15 interleukin 15 [ Mus musculus ]
• Official Symbol: IL15
• Synonyms: IL15; interleukin 15; interleukin-15; IL-15; AI503618
• Gene ID: 16168
• mRNA Refseq: NM_001254747
• Protein Refseq: NP_001241676