Recombinant MultiDsk Ubiquitin Binding Protein, GST-tagged

• Cat.No.: DSK2-01S
• Product Overview: MultiDsk is a novel ubiquitin-binding protein reagent and is composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to GST.
  Highlights:
  Binds ubiquitinated substrates with unprecedented avidity (higher than GST-Dsk2 and TUBE-I)
  GST-fusion allows for use with glutathione agarose beads as an affinity resin to study protein ubiquitination
  Effectively protects ubiquitinated proteins from the action of de-ubiquitylating enzymes and the proteasome in crude cell extracts
  Protective role at concentrations as low as 0.2 µM, making it suitable for use in large-scale extract experiments
  Can be used to efficiently purify both mono- and poly-ubiquitinated proteins
  Flexible linker between individual UBA domains allows free rotation of domains to accommodate multiple Ub chain topologies.
  Highly stable: Can tolerate high salt and detergent conditions whilst still binding
  Highly specific: Does not bind Ubiquitin-like Modifiers (UBLs) SUMO and NEDD8.

Specification

• Description: Enables protein-macromolecule adaptor activity. Involved in protein localization to vacuole; spindle pole body duplication; and ubiquitin-dependent ERAD pathway. Predicted to be located in nucleus. Predicted to be active in cytosol. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis and amyotrophic lateral sclerosis type 15. Orthologous to several human genes including UBQLN2 (ubiquilin 2).
  Ubiquitination is a highly diverse and complex post-translational modification for the regulation of protein function and stability. Studies of ubiquitination have, however, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiquitylating enzymes (DUBs). MultiDsk allows for the capture of pure, native, nontagged, ubiquitinated proteins from cell extracts whilst helping ensure the maintenance of the normal ubiquitination state via a strong protective function, inhibiting de-ubiquitination.
• Source: E. coli, BL-21 (DE3)
• Species: S. cerevisiae
• Tag: GST
• Molecular Mass: 60 kDa including tags
• Applications: Isolation or protection of ubiquitinated proteins in crude cell lysates
• Storage: 4 centigrade for up to 2 weeks (-20 or -80 centigrade for long term storage)
• Concentration: 0.95 mg/mL
• Storage Buffer: 25mM Tris-HCl pH7.5, 150mM NaCl, 50% glycerol, 2mM beta-mercapto-enthanol
• Shipping: Dry ice

Gene Information

• Gene Name: DSK2 ubiquitin domain-containing protein DSK2 [ Saccharomyces cerevisiae S288C ]
• Official Symbol: DSK2
• Synonyms: DSK2; ubiquitin domain-containing protein DSK2; ubiquitin domain-containing protein DSK2; Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; K48-Ub chain binding protein that also binds K63-linked chains in vitro; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; contains an N-terminal Ub-like (UBL) and a C-terminal Ub-associated (UBA) domain; protein abundance increases in response to DNA replication stress
• Gene ID: 855319
• mRNA Refseq: NM_001182783
• Protein Refseq: NP_014003
• UniProt ID: P48510