Recombinant Rhesus CD1A & B2M protein(Met1-Ser299 & Met1-Met119), His-tagged

• Cat.No.: CD1A & B2M-1154R
• Product Overview: Recombinant Rhesus CD1A(NP_001139290.1) (Met1-Ser299) of was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Rhesus B2M (NP_001040602.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the Rhesus CD1C/B2M heterodimer was purified in HEK293.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: C-His
• Protein length: Met1-Ser299 & Met1-Met119
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of rhesus CD1A&B2M comprises 391 (292+99) amino acids and has a calculated molecular mass of 45.2 (33.6+11.6) KDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.