Unactive Recombinant Full Length Human MAPKAPK5 Protein, N-His-tagged

• Cat.No.: MAPKAPK5-162HFL
• Product Overview: N-terminal 6His-tagged, recombinant full-length, human PRAK expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Protein Serine/Threonine Kinase MAPKAPK5 (mitogen-activated protein kinase activated protein kinase 5), also known as p38-regulated/activated protein kinase (PRAK), functions downstream of p38a and p38b in mediating signaling of the p38 pathway. This kinase can be activated in response to cellular stress and proinflammatory cytokines through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 is localized predominantly in the cytoplasm and is constantly shuttled between the cytoplasm and the nucleus. MAPKAPK5 may play a role in balancing other MAPK pathways because over-activation of MAPKAPK5 can inhibit Ras mediated cell proliferation and gene activation. MAPKAPK5 inhibition may facilitate cell differentiation, inflammation and cell proliferation. EMBL AF032437. Protein is R291E with respect to this accession.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.924mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Liquid at -20C.
• Bio-activity: As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with SAPK2a, active.
• Molecular Mass: 55 kDa
• AA Sequence: 1 MHHHHHHMSE ESDMDKAIKE TSILEEYSIN WTQKLGAGIS GPVRVCVKKS TQERFALKIL 61 LDRPKARNEV RLHMMCATHP NIVQIIEVFA NSVQFPHESS PRARLLIVME MMEGGELFHR 121 ISQHRHFTEK QASQVTKQIA LALRHCHLLN IAHRDLKPEN LLFKDNSLDA PVKLCDFGFA 181 KIDQGDLMTP QFTPYYVAPQ VLEAQRRHQK EKSGIIPTSP TPYTYNKSCD LWSLGVIIYV 241 MLCGYPPFYS KHHSRTIPKD MRRKIMTGSF EFPEEEWSQI SEMAKDVVRK LLKVKPEERL 301 TIEGVLDHPW LNSTEALDNV LPSAQLMMDK AVVAGIQQAH AEQLANMRIQ DLKVSLKPLH 361 SVNNPILRKR KLLGTKPKDS VYIHDHENGA EDSNVALEKL RDVIAQCILP QAGENEDEKL 421 NEVMQEAWKY NRECKLLRDT LQSFSWNGRG FTDKVDRLKL AEIVKQVIEE QTTSHESQ
• Purity: Purity 94.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAPKAPK5 MAPK activated protein kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAPKAPK5
• Synonyms: MAPKAPK5; MAPK activated protein kinase 5; MK5; MK-5; NCFD; PRAK; MAPKAP-K5
• Gene ID: 8550
• mRNA Refseq: NM_003668.4
• Protein Refseq: NP_003659.2
• MIM: 606723
• UniProt ID: Q8IW41