| Description : |
Recombinant human MAPKAP Kinase 2 (MAPKAP-K2), amino acids 46-400, A399G, containing an N-terminal GST tag. MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis. The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
46-end |
| Form : |
0.914mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM DTT. Liquid at -20 centigrade. |
| Bio-activity : |
As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with MAP Kinase 2. |
| Molecular Mass : |
70.2 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPGIS GGGGGILEAT 241 MEFHVKSGLQ IKKNAIIDDY KVTSQVLGLG INGKVLQIFN KRTQEKFALK MLQDCPKARR 301 EVELHWRASQ CPHIVRIVDV YENLYAGRKC LLIVMECLDG GELFSRIQDR GDQAFTEREA 361 SEIMKSIGEA IQYLHSINIA HRDVKPENLL YTSKRPNAIL KLTDFGFAKE TTSHNSLTTP 421 CYTPYYVAPE VLGPEKYDKS CDMWSLGVIM YILLCGYPPF YSNHGLAISP GMKTRIRMGQ 481 YEFPNPEWSE VSEEVKMLIR NLLKTEPTQR MTITEFMNHP WIMQSTKVPQ TPLHTSRVLK 541 EDKERWEDVK EEMTSALATM RVDYEQIKIK KIEDASNPLL LKRRKKARAL EAAALGHMEQ 601 KLISEEDLK |
| Purity : |
Purity 78.7% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
