Active Recombinant Full Length Human ACP1 Protein, N-GST-tagged

• Cat.No.: ACP1-93HFL
• Product Overview: N-terminal GSTtagged, recombinant, human LMPTP-A, full length, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: LMPTP-A belongs to the phosphotryosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 2.075mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 10% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 8243U/mg, where one unit of LMPTP-A activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) at room temperature.
• Molecular Mass: 44.5 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAEQ ATKSVLFVCL 241 GNICRSPIAE AVFRKLVTDQ NISENWRVDS AATSGYEIGN PPDYRGQSCM KRHGIPMSHV 301 ARQITKEDFA TFDYILCMDE SNLRDLNRKS NRVKTCKAKI ELLGSYDPQK QLIIEDPYYG 361 NDSDFETVYQ QCVRCCRAFL EKAH
• Purity: Purity 94.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ACP1 acid phosphatase 1 [ Homo sapiens (human) ]
• Official Symbol: ACP1
• Synonyms: ACP1; acid phosphatase 1; HAAP; LMWPTP; LMW-PTP
• Gene ID: 52
• mRNA Refseq: NM_004300.4
• Protein Refseq: NP_004291.1
• MIM: 171500
• UniProt ID: P24666