Description : |
LMPTP-B (Low Molecular Weight Protein Tyrosine Phosphatase B) is a type of protein phosphatase that specifically targets tyrosine residues in proteins. Phosphatases are enzymes that catalyze the removal of phosphate groups from proteins, and this dephosphorylation event can regulate the activity, localization, and interactions of proteins in a cell. The primary function of LMPTP-B is to dephosphorylate tyrosine residues in proteins that are involved in cell signaling pathways. This includes receptor tyrosine kinases (RTKs), which are a class of cell surface receptors that activate signaling pathways when they bind to ligands, and cytoplasmic tyrosine kinases such as Src, which are involved in downstream signaling pathways. Through dephosphorylation of tyrosine residues, LMPTP-B can modulate various cellular processes, including cell proliferation, differentiation, and survival. |
Source : |
E. coli |
Species : |
Human |
Tag : |
GST |
Protein length : |
Full Length |
Form : |
3.777mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : |
13500U/mg, where one unit of LMPTP-B activity is defined as the release of 1nmol of phosphate per minute from the phosphorylated substrate 6,8-difluoro-4- methylumbelliferyl phosphate (DiFMUP) at room temperature. |
Molecular Mass : |
44 kDa |
AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMAEQ ATKSVLFVCL 241 GNICRSPIAE AVFRKLVTDQ NISENWVIDS GAVSDWNVGR SPDPRAVSCL RNHGIHTAHK 301 ARQITKEDFA TFDYILCMDE SNLRDLNRKS NQVKTCKAKI ELLGSYDPQK QLIIEDPYYG 361 NDSDFETVYQ QCVRCCRAFL EKAH |
Purity : |
Purity 100% by SDS-PAGE and Coomassie blue staining. |
Applications : |
Drug Discovery & Development |
Storage : |
1 year at -70 centigrade. |
Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |