Active Recombinant Full Length Human AKT1 Protein, N-His-tagged

• Cat.No.: AKT1-16HFL
• Product Overview: N-terminal 6His-tagged, recombinant, full length, human Akt1, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose

Specification

• Description: PKB (also known as c-Akt or Rac-PK) was originally identified as the transforming oncogene in a retrovirus from a spontaneous thymoma in an AKR mouse. PKB appears to be activated as a consequence of increased phosphoinositide 3-kinase (PI3K) activity in cells stimulated with mitogens. Potential targets of PKB include glycogen synthase kinase-3 and p70 ribosomal protein S6 kinase. PKB is also crucial in mediating cell survival. Activation forms of both PI3 kinase and PKB have been shown to protect from apoptosis induced by withdrawal of growth and survival factors, expression of c-myc, and detachment from extracellular matrix. GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This is reported in EMBL BE 206796.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.803mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 270mM sucrose. Frozen solution.
• Bio-activity: 674U/mg, where one unit of Akt1 activity is defined as 1nmol phosphate incorporated into 30μM modified Crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 59.1 kDa
• AA Sequence: 1 MSFFHHHHHH DFDIPTTENL YFQGAMGSMS DVAIVKEGWL HKRGEYIKTW RPRYFLLKND 61 GTFIGYKERP QDVDQREAPL NNFSVAQCQL MKTERPRPNT FIIRCLQWTT VIERTFHVET 121 PEEREEWTTA IQTVADGLKK QEEEEMDFRS GSPSDNSGAE EMEVSLAKPK HRVTMNEFEY 181 LKLLGKGTFG KVILVKEKAT GRYYAMKILK KEVIVAKDEV AHTLTENRVL QNSRHPFLTA 241 LKYSFQTHDR LCFVMEYANG GELFFHLSRE RVFSEDRARF YGAEIVSALD YLHSEKNVVY 301 RDLKLENLML DKDGHIKITD FGLCKEGIKD GATMKTFCGT PEYLAPEVLE DNDYGRAVDW 361 WGLGVVMYEM MCGRLPFYNQ DHEKLFELIL MEEIRFPRTL GPEAKSLLSG LLKKDPKQRL 421 GGGSEDAKEI MQHRFFAGIV WQHVYEKKLS PPFKPQVTSE TDTRYFDEEF TAQMITITPP 481 DQDDSMECVD SERRPHFPQF SYSASGTA
• Purity: Purity 97.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AKT1 AKT serine/threonine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: AKT1
• Synonyms: AKT1; AKT serine/threonine kinase 1; AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA
• Gene ID: 207
• mRNA Refseq: NM_001014431.2
• Protein Refseq: NP_001014431.1
• MIM: 164730
• UniProt ID: P31749