Active Recombinant Full Length Human BLK Protein, N-His-tagged

• Cat.No.: BLK-25HFL
• Product Overview: N-terminal 6His-tagged, recombinant full-length human Blk, expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Activation of B cells by various ligands is accompanied by activation of Src-family protein tyrosine kinases. Cross-linking of the B-cell receptor leads to the activation of Src-family PTKs BLK (B-cell lymphocyte kinase), Fyn, and Lyn. Specific B and T cell progenitor subsets are preferentially susceptible to transformation by BLK and suggest a role for BLK in the control of proliferation during B cell development. BLK protein tyrosine kinase is found primarily in hematopoietic cells and inhibitors may be of therapeutic use in the treatment of B cell lymphoma.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.81mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 152U/mg, where one unit of Blk, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 58.8 kDa
• AA Sequence: 1 MHHHHHHMGL VSSKKPDKEK PIKEKDKGQW SPLKVSAQDK DAPPLPPLVV FNHLTPPPPD 61 EHLDEDKHFV VALYDYTAMN DRDLQMLKGE KLQVLKGTGD WWLARSLVTG REGYVPSNFV 121 ARVESLEMER WFFRSQGRKE AERQLLAPIN KAGSFLIRES ETNKGAFSLS VKDVTTQGEL 181 IKHYKIRCLD EGGYYISPRI TFPSLQALVQ HYSKKGDGLC QRLTLPCVRP APQNPWAQDE 241 WEIPRQSLRL VRKLGSGQFG EVWMGYYKNN MKVAIKTLKE GTMSPEAFLG EANVMKALQH 301 ERLVRLYAVV TKEPIYIVTE YMARGCLLDF LKTDEGSRLS LPRLIDMSAQ IAEGMAYIER 361 MNSIHRDLRA ANILVSEALC CKIADFGLAR IIDSEYTAQE GAKFPIKWTA PEAIHFGVFT 421 IKADVWSFGV LLMEVVTYGR VPYPGMSNPE VIRNLERGYR MPRPDTCPPE LYRGVIAECW 481 RSRPEERPTF EFLQSVLEDF YTATERQYEL QP
• Purity: Purity 87.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BLK BLK proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BLK
• Synonyms: BLK; BLK proto-oncogene, Src family tyrosine kinase; MODY11
• Gene ID: 640
• mRNA Refseq: NM_001715.3
• Protein Refseq: NP_001706.2
• MIM: 191305
• UniProt ID: P51451