Recombinant Human BLK Protein, His-tagged

• Cat.No.: BLK-01H
• Product Overview: Recombinant human BLK protein (1-505aa) was expressed with N-terminal His-tag in High-Five cells using baculovirus expression system and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 59.8 kDa (525aa)
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGLVS SKKPDKEKPIKEKDKGQWSPLKVSA QDKDAPPLPPLVVFNHLTPPPPDEH LDEDKHFVVALYDYTAMNDRDLQML KGEKLQVLKGTGDWWLARSLVTGRE GYVPSNFVARVESLEMERWFFRSQG RKEAERQLLAPINKAGSFLIRESET NKGAFSLSVKDVTTQGELIKHYKIR CLDEGGYYISPRITFPSLQALVQHY SKKGDGLCQRLTLPCVRPAPQNPWA QDEWEIPRQSLRLVRKLGSGQFGEV WMGYYKNNMKVAIKTLKEGTMSPEA FLGEANVMKALQHERLVRLYAVVTK EPIYIVTEYMARGCLLDFLKTDEGS RLSLPRLIDMSAQIAEGMAYIERMN SIHRDLRAANILVSEALCCKIADFG LARIIDSEYTAQEGAKFPIKWTAPE AIHFGVFTIKADVWSFGVLLMEVVT YGRVPYPGMSNPEVIRNLERGYRMP RPDTCPPELYRGVIAECWRSRPEER PTFEFLQSVLEDFYTATERQYELQP
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 80% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 7.5) containing 50% glycerol, 0.25M NaCl, 5mM DTT, 0.1mM PMSF, 0.1mM EDTA

Gene Information

• Gene Name: BLK BLK proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BLK
• Synonyms: BLK; BLK proto-oncogene, Src family tyrosine kinase; MODY11; tyrosine-protein kinase Blk; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; b lymphocyte kinase; p55-Blk; EC 2.7.10.2
• Gene ID: 640
• mRNA Refseq: NM_001715
• Protein Refseq: NP_001706
• MIM: 191305
• UniProt ID: P51451