| Description : |
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.88mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol. Frozen solution. |
| Bio-activity : |
48U/mg, where one unit of BTK (E41K) activity is defined as 1nmol phosphate incorporated into 300μM LKBtide (LSNLYHQGKFLQTFCGSPLYRRR) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
78.4 kDa |
| AA Sequence : |
1 MHHHHHHEFK GLRRRMAAVI LESIFLKRSQ QKKKTSPLNF KKRLFLLTVH KLSYYKYDFE 61 RGRRGSKKGS IDVEKITCVE TVVPEKNPPP ERQIPRRGEE SSEMEQISII ERFPYPFQVV 121 YDEGPLYVFS PTEELRKRWI HQLKNVIRYN SDLVQKYHPC FWIDGQYLCC SQTAKNAMGC 181 QILENRNGSL KPGSSHRKTK KPLPPTPEED QILKKPLPPE PAAAPVSTSE LKKVVALYDY 241 MPMNANDLQL RKGDEYFILE ESNLPWWRAR DKNGQEGYIP SNYVTEAEDS IEMYEWYSKH 301 MTRSQAEQLL KQEGKEGGFI VRDSSKAGKY TVSVFAKSTG DPQGVIRHYV VCSTPQSQYY 361 LAEKHLFSTI PELINYHQHN SAGLISRLKY PVSQQNKNAP STAGLGYGSW EIDPKDLTFL 421 KELGTGQFGV VKYGKWRGQY DVAIKMIKEG SMSEDEFIEE AKVMMNLSHE KLVQLYGVCT 481 KQRPIFIITE YMANGCLLNY LREMRHRFQT QQLLEMCKDV CEAMEYLESK QFLHRDLAAR 541 NCLVNDQGVV KVSDFGLSRY VLDDEYTSSV GSKFPVRWSP PEVLMYSKFS SKSDIWAFGV 601 LMWEIYSLGK MPYERFTNSE TAEHIAQGLR LYRPHLASEK VYTIMYSCWH EKADERPTFK 661 ILLSNILDVM DEES |
| Purity : |
Purity 62% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -20 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| References : |
Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460 |
