Recombinant Human BTK Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: BTK-092H
• Product Overview: BTK MS Standard C13 and N15-labeled recombinant protein (NP_000052) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 76.1 kDa
• AA Sequence: MAAVILESIFLKRSQQKKKTSPLNF KKRLFLLTVHKLSYYEYDFERGRRG SKKGSIDVEKITCVETVVPEKNPPP ERQIPRRGEESSEMEQISIIERFPY PFQVVYDEGPLYVFSPTEELRKRWI HQLKNVIRYNSDLVQKYHPCFWIDG QYLCCSQTAKNAMGCQILENRNGSL KPGSSHRKTKKPLPPTPEEDQILKK PLPPEPAAAPVSTSELKKVVALYDY MPMNANDLQLRKGDEYFILEESNLP WWRARDKNGQEGYIPSNYVTEAEDS IEMYEWYSKHMTRSQAEQLLKQEGK EGGFIVRDSSKAGKYTVSVFAKSTG DPQGVIRHYVVCSTPQSQYYLAEKH LFSTIPELINYHQHNSAGLISRLKY PVSQQNKNAPSTAGLGYGSWEIDPK DLTFLKELGTGQFGVVKYGKWRGQY DVAIKMIKEGSMSEDEFIEEAKVMM NLSHEKLVQLYGVCTKQRPIFIITE YMANGCLLNYLREMRHRFQTQQLLE MCKDVCEAMEYLESKQFLHRDLAAR NCLVNDQGVVKVSDFGLSRYVLDDE YTSSVGSKFPVRWSPPEVLMYSKFS SKSDIWAFGVLMWEIYSLGKMPYER FTNSETAEHIAQGLRLYRPHLASEK VYTIMYSCWHEKADERPTFKILLSN ILDVMDEESTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: BTK Bruton tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: BTK
• Synonyms: BTK; Bruton tyrosine kinase; AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA; tyrosine-protein kinase BTK; B-cell progenitor kinase; Bruton agammaglobulinemia tyrosine kinase; Bruton's tyrosine kinase; agammaglobulinaemia tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; truncated Bruton agammaglobulinemia tyrosine kinase; EC 2.7.10.2
• Gene ID: 695
• mRNA Refseq: NM_000061
• Protein Refseq: NP_000052
• MIM: 300300
• UniProt ID: Q06187