Active Recombinant Full Length Human CAMK2A Protein, N-His-tagged

• Cat.No.: CAMK2A-32HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human CaM kinase IIα full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CAM KINASE IIα belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.54mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 9003U/mg, where one unit of CaM kinase IIα, active activity is defined as 1nmol phosphate incorporated into 250μM KKLNRTLSFAEPG per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 55 kDa
• AA Sequence: 1 MHHHHHHMAT ITCTRFTEEY QLFEELGKGA FSVVRRCVKV LAGQEYAAKI INTKKLSARD 61 HQKLEREARI CRLLKHPNIV RLHDSISEEG HHYLIFDLVT GGELFEDIVA REYYSEADAS 121 HCIQQILEAV LHCHQMGVVH RDLKPENLLL ASKLKGAAVK LADFGLAIEV EGEQQAWFGF 181 AGTPGYLSPE VLRKDPYGKP VDLWACGVIL YILLVGYPPF WDEDQHRLYQ QIKAGAYDFP 241 SPEWDTVTPE AKDLINKMLT INPSKRITAA EALKHPWISH RSTVASCMHR QETVDCLKKF 301 NARRKLKGAI LTTMLATRNF SGGKSGGNKK SDGVKESSES TNTTIEDEDT KVRKQEIIKV 361 TEQLIEAISN GDFESYTKMC DPGMTAFEPE ALGNLVEGLD FHRFYFENLW SRNSKPVHTT 421 ILNPHIHLMG DESACIAYIR ITQYLDAGGI PRTAQSEETR VWHRRDGKWQ IVHFHRSGAP 481 SVLPH
• Purity: Purity 99% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CAMK2A calcium/calmodulin dependent protein kinase II alpha [ Homo sapiens (human) ]
• Official Symbol: CAMK2A
• Synonyms: CAMK2A; calcium/calmodulin dependent protein kinase II alpha; CAMKA; MRD53; MRT63; CaMKIIalpha; CaMKIINalpha
• Gene ID: 815
• mRNA Refseq: NM_001363989.1
• Protein Refseq: NP_001350918.1
• MIM: 114078
• UniProt ID: Q9UQM7