Active Recombinant Full Length Human CHEK1 Protein, N-GST-tagged

• Cat.No.: CHEK1-57HFL
• Product Overview: N-terminal GST tagged, human full length CHK1, expressed in Sf21 insect cells. Purified using glutathioneagarose.

Specification

• Description: Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK1 regulates numerous checkpoint pathways, including S-phase and G2-M checkpoints. Other potential CHK1 functions include replication, chromatin remodeling and DNA repair. CHK1 inhibitors may sensitize tumors to chemotherapy and radiotherapy.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 0.877mg/ml of enzyme in 50mM Tris/HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 687U/mg, where one unit of CHK1 activity is defined as 1nmol phosphate incorporated into 100μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 81.6 kDa
• AA Sequence: 1 MPILGYWKIK GLVQPTRLLL EYLEEKYEEH LYERDEGDKW RNKKFELGLE FPNLPYYIDG 61 DVKLTQSMAI IRYIADKHNM LGGCPKERAE ISMLEGAVLD IRYGVSRIAY SKDFETLKVD 121 FLSKLPEMLK MFEDRLCHKT YLNGDHVTHP DFMLYDALDV VLYMDPMCLD AFPKLVCFKK 181 RIEAIPQIDK YLKSSKYIAW PLQGWQATFG GGDHPPKSDL VPRGSRRASV GSHMAVPFVE 241 DWDLVQTLGE GAYGEVQLAV NRVTEEAVAV KIVDMKRAVD CPENIKKEIC INKMLNHENV 301 VKFYGHRREG NIQYLFLEYC SGGELFDRIE PDIGMPEPDA QRFFHQLMAG VVYLHGIGIT 361 HRDIKPENLL LDERDNLKIS DFGLATVFRY NNRERLLNKM CGTLPYVAPE LLKRREFHAE 421 PVDVWSCGIV LTAMLAGELP WDQPSDSCQE YSDWKEKKTY LNPWKKIDSA PLALLHKILV 481 ENPSARITIP DIKKDRWYNK PLKKGAKRPR VTSGGVSESP SGFSKHIQSN LDFSPVNSAS 541 SEENVKYSSS QPEPRTGLSL WDTSPSYIDK LVQGISFSQP TCPDHMLLNS QLLGTPGSSQ 601 NPWQRLVKRM TRFFTKLDAD KSYQCLKETC EKLGYQWKKS CMNQVTISTT DRRNNKLIFK 661 VNLLEMDDKI LVDFRLSKGD GLEFKRHFLK IKGKLIDIVS SQKVWLPAT
• Purity: Purity 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens (human) ]
• Official Symbol: CHEK1
• Synonyms: CHEK1; checkpoint kinase 1; CHK1; OZEMA21
• Gene ID: 1111
• mRNA Refseq: NM_001114121.2
• Protein Refseq: NP_001107593.1
• MIM: 603078
• UniProt ID: O14757