Active Recombinant Full Length Human CLK3 Protein, N-His-tagged

• Cat.No.: CLK3-60HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length human CLK3, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: This gene encodes a protein belonging to the serine/threonine type protein kinase family. This protein is a nuclear dual-specificity kinase that regulates the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors. Two transcript variants encoding different isoforms have been found for this gene. Related pseudogenes are located on chromosomes 1 and 9.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.028mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1378U/mg, where one unit of CLK3 activity is defined as 1nmol phosphate incorporated into 250μM (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 63 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMHHC KRYRSPEPDP YLSYRWKRRR 61 SYSREHEGRL RYPSRREPPP RRSRSRSHDR LPYQRRYRER RDSDTYRCEE RSPSFGEDYY 121 GPSRSRHRRR SRERGPYRTR KHAHHCHKRR TRSCSSASSR SQQSSKRSSR SVEDDKEGHL 181 VCRIGDWLQE RYEIVGNLGE GTFGKVVECL DHARGKSQVA LKIIRNVGKY REAARLEINV 241 LKKIKEKDKE NKFLCVLMSD WFNFHGHMCI AFELLGKNTF EFLKENNFQP YPLPHVRHMA 301 YQLCHALRFL HENQLTHTDL KPENILFVNS EFETLYNEHK SCEEKSVKNT SIRVADFGSA 361 TFDHEHHTTI VATRHYRPPE VILELGWAQP CDVWSIGCIL FEYYRGFTLF QTHENREHLV 421 MMEKILGPIP SHMIHRTRKQ KYFYKGGLVW DENSSDGRYV KENCKPLKSY MLQDSLEHVQ 481 LFDLMRRMLE FDPAQRITLA EALLHPFFAG LTPEERSFHT SRNPSR
• Purity: Purity 83.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CLK3 CDC like kinase 3 [ Homo sapiens (human) ]
• Official Symbol: CLK3
• Synonyms: CLK3; CDC like kinase 3; PHCLK3; PHCLK3/152
• Gene ID: 1198
• mRNA Refseq: NM_003992.5
• Protein Refseq: NP_003983.2
• MIM: 602990
• UniProt ID: P49761