Active Recombinant Full Length Human FYN Protein, N-His-tagged

• Cat.No.: FYN-70HFL
• Product Overview: N-Terminal 6Histagged, recombinant, full-length, human Fyn, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Fyn is a member of the src-related gene family of non-receptor tyrosine kinases. Recent studies indicate that fin kinase plays a critical role in mediating signal transduction through the T-cell receptor thereby initiating a signal transduction cascade leading to lymphokine secretion and cell proliferation. One of the earliest biochemical responses following T-cell receptor activation is an increase in tyrosine kinase activity and concomitant phosphorylation of several intracellular substrates. Fyn kinase is an important mediator of this phosphorylation since it is physically associated with the T-cell receptor. Additional evidence supports the view that fin participates in signal transduction pathways leading from multiple T-cell membrane proteins and hence may serve to integrate stimuli received from several independent receptor structures. In the case of the B-cell receptor, fin is one of several kinases associated with the receptor and is activated following receptor cross-linking.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.58mg/ml of enzyme in 50mM Tris/HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 5% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1290U/mg, where one unit of Fyn activity is defined as 1nmol phosphate incorporated into 250μM cdc2 peptide per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 61.8 kDa
• AA Sequence: 1 MHHHHHHMGC VQCKDKEATK LTEERDGSLN QSSGYRYGTD PTPQHYPSFG VTSIPNYNNF 61 HAAGGQGLTV FGGVNSSSHT GTLRTRGGTG VTLFVALYDY EARTEDDLSF HKGEKFQILN 121 SSEGDWWEAR SLTTGETGYI PSNYVAPVDS IQAEEWYFGK LGRKDAERQL LSFGNPRGTF 181 LIRESETTKG AYSLSIRDWD DMKGDHVKHY KIRKLDNGGY YITTRAQFET LQQLVQHYSE 241 RAAGLCCRLV VPCHKGMPRL TDLSVKTKDV WEIPRESLQL IKRLGNGQFG EVWMGTWNGN 301 TKVAIKTLKP GTMSPESFLE EAQIMKKLKH DKLVQLYAVV SEEPIYIVTE YMNKGSLLDF 361 LKDGEGRALK LPNLVDMAAQ VAAGMAYIER MNYIHRDLRS ANILVGNGLI CKIADFGLAR 421 LIEDNEYTAR QGAKFPIKWT APEAALYGRF TIKSDVWSFG ILLTELVTKG RVPYPGMNNR 481 EVLEQVERGY RMPCPQDCPI SLHELMIHCW KKDPEERPTF EYLQSFLEDY FTATEPQYQP 541 GENL
• Purity: Purity 61.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: FYN FYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: FYN
• Synonyms: FYN; FYN proto-oncogene, Src family tyrosine kinase; SLK; SYN; p59-FYN
• Gene ID: 2534
• mRNA Refseq: NM_002037.5
• Protein Refseq: NP_002028.1
• MIM: 137025
• UniProt ID: P06241