Active Recombinant Full Length Human GRK1 Protein, N-His-tagged

• Cat.No.: GRK1-71HFL
• Product Overview: N-terminal 6Histagged, recombinant, human GRK1 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: GRK1 is a retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.930mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 94U/mg, where one unit of GRK1 activity is defined as 1nmol phosphate incorporated into 300μM (KKKKERLLDDRHDSGLDSMKDEE) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 68 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF GRRMDFGSLE TVVANSAFIA ARGSFDGSSS 61 QPSRDKKYLA KLKLPPLSKC ESLRDSLSLE FESVCLEQPI GKKLFQQFLQ SAEKHLPALE 121 LWKDIEDYDT ADNDLQPQKA QTILAQYLDP QAKLFCSFLD EGIVAKFKEG PVEIQDGLFQ 181 PLLQATLAHL GQAPFQEYLG SLYFLRFLQW KWLEAQPMGE DWFLDFRVLG KGGFGEVSAC 241 QMKATGKLYA CKKLNKKRLK KRKGYQGAMV EKKILMKVHS RFIVSLAYAF ETKADLCLVM 301 TIMNGGDIRY HIYNVNEENP GFPEPRALFY TAQIICGLEH LHQRRIVYRD LKPENVLLDN 361 DGNVRISDLG LAVELLDGQS KTKGYAGTPG FMAPELLQGE EYDFSVDYFA LGVTLYEMIA 421 ARGPFRARGE KVENKELKHR IISEPVKYPD KFSQASKDFC EALLEKDPEK RLGFRDETCD 481 KLRAHPLFKD LNWRQLEAGM LMPPFIPDSK TVYAKDIQDV GAFSTVKGVA FDKTDTEFFQ 541 EFATGNCPIP WQEEMIETGI FGELNVWRSD GQMPDDMKGI SGGSSSSSKS GMCLVS
• Purity: Purity 84% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GRK1 G protein-coupled receptor kinase 1 [ Homo sapiens (human) ]
• Official Symbol: GRK1
• Synonyms: GRK1; G protein-coupled receptor kinase 1; RK; RHOK; GPRK1
• Gene ID: 6011
• mRNA Refseq: NM_002929.3
• Protein Refseq: NP_002920.1
• MIM: 180381
• UniProt ID: Q15835